从野外种植的花生小结体中分离和描述细菌内细胞
Md Shakhawat Hossain1,2, Christine Frith3, Siddhartha Shankar Bhattacharyya1,2
1Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA.
Microorganisms
|August 26, 2023
概括
花生根结核中含有各种各样的细菌. 一些非树生物内植物表现出植物生长促进 (PGP) 和耐压力,表明可持续农业生物制品的潜力.
科学领域:
- 微生物学 微生物学
- 植物科学 植物科学
- 生物技术是生物技术.
背景情况:
- 豆类根结核可以容纳根菌和非根菌细菌内菌.
- 小花生结核可以含有各种各样的细菌内生菌,促使人们对它们的作用进行调查.
研究的目的:
- 从花生根结核中分离和表征土著细菌内细胞.
- 评估这些内植物的分子,生化和生理特征,包括耐压力和促进植物生长 (PGP) 能力.
主要方法:
- 从野外种植的花生根中分离出87种内植物.
- 使用16S rRNA基因进行了家族遗传学分析.
- 生物化学测定压力耐受性 (温度,盐度,pH值) 和生产氨,醇-3-酸 (IAA),1-aminocyclopropane-1-carboxylate deaminase (ACCD) 和 siderophores.
- 植物和结节测试以评估PGP活动.
主要成果:
- 遗传学分析发现了多种不同的属,包括Bacillus,Burkholderia,Pantoea,Pseudomonas和Rhizobium.
- 大多数分离物 (78%) 对高温,盐度和酸性条件有耐受性.
- 几种分离物体显示出大量的IAA,氨, siderophores,ACC除氨酶和酸盐溶解的产生.
- 选择的分离物,虽然没有结节,但显示出显著的PGP活性,增强了生物质生产.
结论:
- 本土花生根结节内植物具有有利于植物生长和应激弹性的特征.
- 这些内植物具有开发生物制剂的潜力,以增强可持续的作物改进.
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