来自Trichoderma reesei的Cerato-Platanin EPL2不是直接参与细胞酶的形成,而是参与细胞壁重塑
Alinne Costa Silva1, Letícia Harumi Oshiquiri1, Luiz Felipe de Morais Costa de Jesus1
1Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14049-900, SP, Brazil.
Microorganisms
|August 26, 2023
概括
在Trichoderma reesei中删除EPL2基因会影响基因表达,导致对细胞壁压力因素的抗性增加. 这表明EPL2在真菌细胞壁重塑和生物技术应用中起到了补偿作用.
科学领域:
- 生物技术是生物技术.
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
背景情况:
- 体真菌是工业酶生产的关键真菌,特别是纤维素.
- 甲素 (CPs) 是具有多种生理作用的真菌蛋白质,但它们在T. reesei中的特定功能尚不清楚.
- 在T. reesei中存在三个编码CP的基因,包括Trire2_123955 (EPL2),其功能需要阐明.
研究的目的:
- 为了研究Trichoderma reesei中的EPL2蛋白的功能.
- 分析EPL2删除对基因表达和细胞生理学的影响.
- 评估了解EPL2的潜力,以改善T. reesei.的生物技术应用.
主要方法:
- 在野生型T. reesei菌株中删除了Trire2_123955 (epl2) 的基因.
- RNA测序用于比较野生类型和Depl2突变菌株之间的基因表达特征.
- 分析真菌生长,细胞壁组成 (素,葡萄糖) 和抗压力.
主要成果:
- 删除epl2导致T. reesei.中760个基因的差异表达.
- 与氧化应激,氧化还原酶活性,抗氧化活性和传输相关的基因的升级在Depl2突变中.
- 德普尔2突变体表现出和葡萄糖积累增加,对细胞壁压力因素的抗性增强,以及细胞壁合成基因的上调.
结论:
- 在T. reesei中缺少EPL2诱导细胞壁重塑中的补偿机制.
- EPL2在调节真菌细胞壁完整性和应激反应方面发挥作用.
- 了解EPL2的功能可能会提高T. reesei在工业生物技术中的实用性.
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