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玉米ZmBES1/BZR1-9转录因子加速了转基因Arabidopsis和大米的开花

Yuan Liu¹, Hongwanjun Zhang¹, Wenqi Feng¹

¹Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region; Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China.

Plants (Basel, Switzerland)

|August 26, 2023

概括

玉米ZmBES1/BZR1-9基因加速了Arabidopsis和大米的开花时间,分别为10天和8天. 这种转录因子调节了开花时间,并影响了与植物生长和发育有关的基因表达.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
农作物科学农作物科学

背景情况:

铜体信号通路转录因子BES1/BZR1对于模型植物的生长和发育至关重要.
玉米BES1/BZR1 (ZmBES1/BZR1) 成员的特定功能,特别是在花期调节方面,仍然在很大程度上没有特征.

研究的目的:

研究玉米ZmBES1/BZR1-9基因在调节开花时间中的作用.
探索ZmBES1/BZR1-9通过哪些分子机制影响异构系统 (Arabidopsis和大米) 的开花.

主要方法:

在Arabidopsis thaliana (野生类型和bes1-D突变) 和大米 (Oryza sativa) 中ZmBES1/BZR1-9基因的异胎表达.
花开时间的表型分析 (花开的几天,花叶数).
使用RNA测序 (RNA-seq) 和定量实时PCR (qRT-PCR) 的基因表达分析.

主要成果:

在阿拉比多普西斯中,ZmBES1/BZR1-9的过度表达和补充导致了显著更早的开花 (大约. 10天) 没有改变罗塞特叶数.
在大米中ZmBES1/BZR1-9过度表达也导致了加快的标题日期 (约. 8天) 的时间.
表达分析显示了转基因阿拉比多普西斯和大米系中关键的与开花相关基因 (例如At2-MMP,OsGA20OX1) 的上调,以及许多其他基因的差异表达.

结论:

ZmBES1/BZR1-9在双叶植物 (Arabidopsis) 和单叶植物 (大米) 种类中控制开花时间方面发挥着重要作用.
这些发现提供了关于BES1/BZR1转录因子在不同作物物种中调节植物生长和发育,特别是开花的保护功能的见解.

关键词:

在BES1/BZR1中.宫外表达的表达方式花期是花期的花期.监管监管监管监管监管监管植物生长植物生长

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