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提高牛乳酸的细胞吸收和运输,使用素和酸盐修饰的固体脂质纳米颗粒

Xudong Yao¹, Craig Bunt², Mengyang Liu¹

¹School of Pharmacy, Faculty of Medical and Health Science, The University of Auckland, Auckland 1142, New Zealand.

|August 26, 2023

概括

修改的固体脂质纳米颗粒 (SLPs) 有效地增强了牛乳酸 (bLf) 的细胞吸收和运输. 这些bLf装载的SLP显示出改善口服蛋白质生物可用性的潜力.

科学领域:

纳米技术纳米技术
生物制药生物制药公司
材料科学材料科学材料科学

背景情况:

牛乳酸 (bLf) 是一种具有治疗潜力的蛋白质,但在口服中面临着挑战.
固体脂质纳米粒子 (SLPs) 为蛋白质提供了一个有前途的输送系统.
修改SLP的表面可能会增强细胞相互作用和运输.

研究的目的:

为牛乳酸 (bLf) 开发素和奇托桑修饰的固体脂质纳米颗粒 (SLPs).
通过使用Caco-2细胞模型评估bLf装载SLP的体外细胞吸收和运输.
研究SLPs在改善蛋白质口服生物可用性的潜力.

主要方法:

用乙烯酸和溶剂蒸发技术制备 bLf 装载的 SLP.
加入点或奇托桑用于表面修饰.
细胞吸收和运输在Caco-2细胞中的体外评估.
同焦激光扫描显微镜和明显透系数的评估 (应用程序).

主要成果:

bLf装载的SLP没有显著的细胞毒性或亡.
带有bLf的SLP的细胞吸收量明显高于单独的bLf,由内细胞分裂介导.
bLf摄取跟随受体介导的内细胞分裂.
维拉帕米尔和EDTA增强了bLf和SLPs的表面透系数 (App).

结论:

pectin和chitosan的修饰增强了SLP的细胞吸收和运输.
SLP可能利用涉及P-糖蛋白的活性运输机制.
SLPs显示出作为有效载体的潜力,可以提高蛋白质和的口服生物可用性.

关键词:

牛乳酪蛋白是牛乳酪蛋白中的一种.蜂传输是通过蜂传输的方式进行的.细胞吸收细胞吸收.基托桑修饰的固体脂质纳米颗粒

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