酵母细胞壁生物聚合物作为益生菌封装剂的潜在应用
Gemilang Lara Utama1,2, Lidya Oktaviani1, Roostita Lobo Balia3
1Faculty of Agro-Industrial Technology, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia.
Polymers
|August 26, 2023
概括
酵母细胞壁生物聚合物如β-葡萄糖和曼诺蛋白可以封装益生菌. 这些天然化合物增强了益生菌的生存和功能,为同生菌的发展提供了潜力.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
背景情况:
- 酵母细胞壁生物聚合物,包括β-葡萄糖,曼蛋白和基,是益生菌的潜在封装剂.
- β-葡萄糖在冷干燥过程中提供了冷保护的热稳定性.
- 曼诺蛋白增强益生菌对肠道细胞的附着性,并且素经常被用作素.
研究的目的:
- 探索酵母细胞壁生物聚合物的提取和净化,用于益生菌封装.
- 评估这些生物聚合物在增强益生菌生存和功能方面的潜力.
主要方法:
- 优化从酒厂废物中提取β-葡萄糖,使用热解和超声波.
- 使用酶处理和沉来分离和净化曼诺蛋白.
- 酸反应,透析和冷化以提取基.
- 染色学技术 (DEAE离子交换,康卡纳瓦林-A,亲缘关系) 用于生物聚合物净化.
主要成果:
- 获得了高度的β-葡萄糖 (高达14%) 和纯化的曼诺蛋白 (7.25%).
- 通过一种特殊的酸过程,获得了2.4%的最大基产量.
- 净化方法显著提高了单个生物聚合物的纯度.
- 生物聚合物在促进益生菌生存和形成共生菌方面已证明其潜力.
结论:
- 酵母细胞壁生物聚合物是益生菌的有效封装剂和功能成分.
- 优化的提取和净化方法产生高纯度的生物聚合物.
- 这些生物聚合物增强了益生菌的生存能力,粘附性,并可以形成有益的共生菌.
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