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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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无标签的DNA检测使用蚀刻倾斜布拉格纤维格式基于生物传感器.

Abdullah Al Noman1, Jitendra Narayan Dash2, Md Abdullah Al Maruf3

  • 1Department of Electronic and Information Engineering, Photonics Research Institute, The Hong Kong Polytechnic University, 11 Yuk Choi Rd, Hung Hom, Hong Kong SAR, China.

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概括

这项研究引入了一种新的光纤生物传感器,使用雕刻的倾斜纤维布拉格格 (TFBG) 进行无标签DNA检测. 开发的生物传感器对补充DNA (cDNA) 检测具有很高的灵敏度和特异性.

关键词:
它们是DNA DNA DNA DNA.生物传感器生物传感器蚀刻 蚀刻 蚀刻 是一种方法.光纤传感器是一种光纤传感器.倾斜的纤维布拉格格子 布拉格格子

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科学领域:

  • 光电学是指光电子产品.
  • 生物技术是生物技术.
  • 纳米材料是一种纳米材料.

背景情况:

  • 光纤生物传感器提供无标签检测能力.
  • 倾斜纤维布拉格格 (TFBGs) 是生物传感应用的敏感光学结构.
  • 检测DNA杂交对诊断和研究至关重要.

研究的目的:

  • 提出和展示一个无标签的光纤生物传感器.
  • 为了利用一个蚀刻的TFBG功能,用DNA探针进行敏感检测.
  • 为了评估传感器的灵敏度,检测极限,可重现性和特异性.

主要方法:

  • 使用相面罩刻入倾斜纤维布拉格格 (TFBG).
  • 用酸对TFBG进行蚀刻.
  • 沉积一个聚乙烯胺 (PEI) /多烯酸 (PAA) 复合层.
  • 将探针DNA (pDNA) 固定在功能化的TFBG.上.
  • 通过波长依赖的询问来监测DNA杂交.

主要成果:

  • 制造的生物传感器证明了对辅助DNA (cDNA) 的无标签检测.
  • 在三个相同的探测器中证实了可重复性.
  • 获得了320 pm/μM的最大灵敏度.
  • 检测极限被确定为0.65μM.
  • 对非目标DNA序列观察到高特异性.

结论:

  • 嵌入式基于TFBG的光纤生物传感器是无标签DNA检测的可行平台.
  • 传感器具有出色的灵敏度,低检测极限和高特异性.
  • 这项技术具有各种诊断和研究应用的潜力.