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聚合物输送系统作为潜在的针对内脏莱什曼病的疫苗:配方开发和免疫性

João Guilherme Lino da Silva^1,2, Ana Alice Maia Gonçalves¹, Liliam Teixeira Oliveira³

¹Laboratory of Biology of Cell Interactions, Department of Morphology, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, Brazil.

|August 26, 2023

概括

酸盐涂层的微小粒子 (SMPs) 显示出作为抗莱什马尼亚疫苗的辅助剂的潜力. 这些颗粒刺激天生的免疫反应而不会引起毒性,这表明它们可以增强疫苗的免疫性.

科学领域:

免疫学免疫学免疫学
疫苗开发疫苗开发
材料科学材料科学材料科学

背景情况:

提高疫苗免疫性对于有效预防疾病至关重要.
目前正在探索基于微粒的传递系统,以改善抗原呈现和免疫反应.
用素涂层的多D,L-乳酸亚微米颗粒 (SMPs) 被研究其辅助性质.

研究的目的:

评估基托涂层SMPs对Leishmania抗原的局部辅助作用.
分析SMP配方的炎症概况和毒性.
评估SMPs对免疫细胞群体在接种疫苗后的影响.

主要方法:

制备了两种SMP配方 (SMP1和SMP2),含有不同度的多D,L-乳酸 (PLA) 和奇托.
给小鼠服用未加载的SMP,以评估最初的炎症反应 (细胞招募,MPO,NAG,IL-6,TNF-α水平).
Leishmania braziliensis抗原被封装在SMP中,接种疫苗的小鼠使用流细胞计分析免疫性.

主要成果:

没有装载的SMP促进了细胞招募,而不会造成组织损伤,并降低了NAG水平.
SMPs诱导可检测的TNF-α水平,表明炎症反应.
用带有抗原的SMPs接种疫苗显示免疫细胞概况发生变化,SMP2对T细胞和B细胞的影响更为显著.

结论:

桑涂层的SMP配方有效地刺激早期的先天免疫反应.
这些SMP显示出作为辅助剂的潜力,以增强Leishmania疫苗的免疫性.
需要进一步的研究来优化SMP配方,以提高疫苗的有效性和安全性.

关键词:

聚合物纳米粒子的聚合物纳米粒子.亚微米粒子是微小的粒子.疫苗疫苗疫苗疫苗有关内脏的莱什曼病.

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