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免疫接种和宿主对MB-1的反应,MB-1是一种活体化场疫苗,用于预防传染病
Yossi Wein1, Virginie Loeb2, Aderajew Asmare2
1Department of Animal Sciences, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 7610001, Israel.
孕产妇产生的抗体延迟了小的MB-1疫苗病毒复制. 脏充当病毒的储存库,直到母亲的抗体下降,使囊殖民和有效免疫感染性囊病.
科学领域:
- 兽医病毒学 兽医病毒学
- 免疫学 免疫学 免疫学
- 鸟类病理学 鸟类病理学
背景情况:
- 孕产妇产生的抗体 (MDAs) 可以干扰疫苗的疗效.
- 在MDA中和期间疫苗病毒存活的机制尚未完全理解.
- MB-1是一种已知表现出延迟复制的弱化传染病病菌病毒 (IBDV) 疫苗.
研究的目的:
- 阐明MB-1疫苗病毒在小中复制的时间延迟背后的机制.
- 调查巨细胞和淋巴体器官在MDA存在期间MB-1病毒存活中的作用.
- 了解MB-1如何在MDA干扰的情况下成功实现免疫接种.
主要方法:
- 用MB-1疫苗对1天大小的小进行接种 (DOA).
- 在接种疫苗后的不同时间点对淋巴体器官 (脏和毛囊) 的PCR分析.
- 在不同组织中量化MB-1病毒水平.
主要成果:
- 在从1 DOA到14天的小的脏中检测到低水平的MB-1病毒.
- 疫苗接种后的14天内,病毒在囊中的水平保持在低水平,然后增加.
- 在MDA水平高的时期,脏充当病毒储存库.
- 在囊间隙中存在抗IBDV囊VP2母体IgA和IgY,可能延迟了殖民.
结论:
- MB-1病毒通过居住在巨细胞中并在脏中建立储存库来幸存MDA中和.
- 基于脏的水库允许在MDA水平下降后延迟殖民囊.
- 这种机制保证了最终成功免疫接种传染性病 (Gumboro病).
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