多个猪先天性免疫信号通路参与了反PEDV反应
Youwen Zhang1,2,3,4, Yulin Xu1,2,3,4, Sen Jiang1,2,3,4
1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Viruses
|August 26, 2023
概括
猪流行性腹病毒 (PEDV) 感染受到多种先天免疫通路的显著抑制. TRIF,MAVS和STING信号对于控制猪PEDV复制至关重要.
科学领域:
- 兽医病毒学 兽医病毒学
- 天生的免疫学天生的免疫学
- 分子生物学分子生物学
背景情况:
- 猪流行性腹病毒 (PEDV) 在全球猪业造成了巨大的经济损失.
- 天生的免疫在对抗PEDV感染中的确切作用仍然不完全理解.
- 了解抗病毒机制对于缓解PEDV爆发至关重要.
研究的目的:
- 调查猪天生的免疫信号适配器在抗PEDV活动中的参与.
- 阐明TRIF,MAVS和STING在对PEDV的先天免疫反应中的特定作用.
- 提供对PEDV控制的新型抗病毒策略的见解.
主要方法:
- 感染Vero细胞与天生的免疫信号适配器.
- 使用类似AJ1102菌株对抗PEDV活性的评估.
- 激素激应和siRNA敲击测试以评估TRIF,MAVS和STING功能.
- 在CRISPR-Cas9基因编辑中创建TRIF,MAVS和STING淘汰Vero细胞.
- 在猪肠上皮细胞 (IPEC-DQ) 中进行验证.
主要成果:
- 在转染的Vero细胞中,TRIF和MAVS显示出显著的抗PEDV活性.
- 内源的TRIF,MAVS和STING信号通路表现出明显的抗PEDV效应.
- 淘汰和淘汰研究证实了TRIF,MAVS和STING在限制PEDV复制中的参与.
- 这些发现可以在猪IPEC-DQ细胞中复制.
结论:
- 多种模式识别受体 (PRR) 信号通路,包括TRIF,MAVS和STING,是猪对PEDV的天生的免疫反应的关键组成部分.
- 这项研究增强了我们对PEDV感染相关的抗病毒机制的理解.
- 鉴定的途径为开发新的策略提供了潜在的目标,以预防和控制PEDV的传播.
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