由DNMT1抑制诱导的宿主DNA脱甲基化 在流感病毒感染期间对抗病毒OASL蛋白进行上调调节
Zhiyan Zhao1,2, Jing Li2, Ye Feng2
1School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China.
Viruses
|August 26, 2023
概括
流感A病毒感染通过影响DNMT1表达来改变DNA甲基化,导致OASL蛋白增加. 这增强了天生的免疫力,抑制了病毒复制,为宿主抗病毒防御提供了洞察力.
科学领域:
- 病毒学 病毒学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 免疫学 免疫学 免疫学
背景情况:
- 甲型流感病毒 (IAV) 导致人类严重的呼吸系统疾病.
- IAV感染会影响DNA甲基转移酶 (DNMTs),通过DNA甲基化改变基因表达.
- IAV诱导的表观遗传变化的机制及其在宿主相互作用中的作用尚未完全理解.
研究的目的:
- 阐明IAV感染背后的表观遗传机制.
- 调查DNA甲基化在IAV与宿主相互作用中的作用.
- 为了了解宿主抗病毒反应在IAV感染期间是如何调节的.
主要方法:
- 在IAV感染的A549细胞中抑制miR-142-5p和PI3K/AKT信号通路.
- 对DNA甲基转移酶1 (DNMT1) 表达的分析.
- 评估2'-5'-奥利戈阿丁烯酸酸类似合成酶 (OASL) 蛋白质促进剂去甲基化和表达.
- 对RIG-I介导的干扰素诱导和IAV复制的评估.
主要成果:
- IAV感染在抑制miR-142-5p或PI3K/AKT通路时抑制了DNMT1的表达.
- 这种抑制导致了OASL促进物的去甲基化,并增加了OASL的表达.
- OASL上调增强了RIG-I介导的干扰素诱导.
- 增加的OASL表达导致抑制的IAV复制.
结论:
- IAV感染引发了涉及DNMT1和miR-142-5p/PI3K/AKT通路的表观遗传修饰.
- 通过表观遗传变化的OASL的升级增强了对IAV的先天免疫力.
- 这项研究揭示了一种新的宿主抗病毒机制,涉及IAV感染期间OASL的表观遗传调节.
相关概念视频
Leaky Scanning
5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K
siRNA - Small Interfering RNAs
16.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.8K
Viruses with RNA Genomes
55
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
55
RNA Interference
26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K
Immune Response Against Viral Pathogens
824
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
824
Experimental RNAi
6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K


