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抑制马雷克病病毒复制和传播的25-基胆固醇和27-基胆固醇在体外

Nitin Kamble¹, Vishwanatha R A P Reddy¹, Ben Jackson¹

¹Avian Immunology Group, The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NE, UK.

|August 26, 2023

概括

在中马雷克病病毒 (MDV) 感染可以通过25-胆固醇 (25-HC) 和27-胆固醇 (27-HC) 控制. 这些由胆固醇衍生的化合物显示出对MDV复制的抗病毒药物的潜力.

科学领域:

兽医病毒学兽医病毒学
免疫学免疫学免疫学
生物化学生物化学

背景情况:

马雷克病病毒 (MDV) 由于淋巴增殖性疾病,在家禽行业造成重大经济损失.
众所周知,MDV通过抑制I型干扰素诱导来逃避免疫反应.

研究的目的:

研究胆固醇代谢物25-胆固醇 (25-HC) 和27-胆固醇 (27-HC) 在控制MDV感染中的作用.
确定I型干扰素 (IFN-α) 是否影响参与25-HC和27-HC合成的基因的表达.

主要方法:

露西法酶测定检测类型I IFN (IFN-α) 反应后的MDV感染.
用RT-PCR分析胚胎纤维细胞 (CEF) 中CH25H和CYP27A1基因的上调调.
标准斑块检测评估25-HC和27-HC对MDV的抗病毒作用.

主要成果:

患有MDV的感染最初刺激了IFN-α,但随后在以后的时间点出现了显著的减少.
类型I IFN (IFN-α) 在CEF细胞中调高了CH25H和CYP27A1基因表达.
使用25-HC或27-HC的治疗在体外显著降低了MDV标位和斑块大小.

结论:

25-HC和27-HC对马雷克病病毒表现出抗病毒活性.
这些胆固醇代谢物显示出作为控制家禽中MDV复制和传播的潜在治疗剂的前景.

关键词:

25氧胆固醇是25-氧胆固醇的一种.这是一种27-基胆固醇.CH25H是指CH25H的含量.在 CYP27A1 中.通过IFN刺激的基因.马雷克的疾病病毒病毒.病毒复制是病毒的复制.

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