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从印度安得拉邦的症状病例中分离出来的SARS-CoV-2变种的突变分析

Mudhigeti Nagaraja¹, Kodavala Sireesha², Anagoni Srikar¹

¹State-Level VRDL, Department of Clinical Virology, Sri Venkateswara Institute of Medical Sciences, Tirupati 517 507, Andhra Pradesh, India.

|August 26, 2023

概括

由于基因组进化,监测印度的SARS-CoV-2变种至关重要. 这项研究确定了德尔塔是安得拉邦的主要变种,其次是Omicron,分析了尖峰基因突变.

科学领域:

病毒学和分子流行病学.
病毒病原体的基因组监测
公共卫生基因组学

背景情况:

由于SARS-CoV-2基因组的不断演变,因此需要持续监测循环的变种.
了解区域变种流行率对于有效的公共卫生战略和流行病应对至关重要.

研究的目的:

在印度安得拉邦检测和描述SARS-CoV-2变种.
分析尖端基因突变并将其与全球流行数据进行比较.

主要方法:

使用桑格测序从104个临床样本中测序完整的S基因.
使用Nextclade和GISAID工具对核酸和氨基酸突变的分析.
对完整的编码序列 (CDS) 的遗传学分析,以确定变异类.

主要成果:

德尔塔变种是最常见的 (38/104),其次是Omicron (31/104).
在尖峰基因中发现了69种不同的氨基酸替代物,其中T95I主要存在于Delta变异中.
遗传学分析将样本放置在包括阿尔法,三角洲,卡帕和奥米克朗在内的各种类别中.

结论:

该研究成功地在印度安得拉邦特征了SARS-CoV-2变种,突出了三角洲和Omicron的统治地位.
鉴定到的突变,特别是在Omicron变种的furin分裂部位附近,可能会影响病毒的毒性和传播.
基因组监测为跟踪病毒演变和告知公共卫生干预提供了重要的数据.

关键词:

安得拉邦安得拉邦印度印度印度在S基因测序过程中,S基因测序这就是SARS-CoV-2病毒.氨基酸替代物的氨基酸替代物循环叶片是循环的叶片.突变是发生在突变中的突变.

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