芬兰和瑞典的阿斯马赛克相关病毒的分子种群遗传学

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Shaheen Nourinejhad Zarghani¹, Rim Al Kubrusli¹, Serghei Iancev¹

¹Division Phytomedicine, Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt-Universität zu Berlin, Lentzeallee 55-57, 14197 Berlin, Germany.

|August 26, 2023

概括

阿斯马赛克相关病毒 (AsMaV) 表现出遗传多样性和地理结构,特别是在瑞典和芬兰的分离物之间. 其保存的基因组,在净化选择下,为管理树疾病提供了洞察力.

科学领域:

病毒学和植物病理学
分子生物学和基因组学
生态学和进化生物学.

背景情况:

阿斯木马赛克相关病毒 (AsMaV),一种埃马拉病毒,在阿斯树 (Populus tremula) 中引起马赛克症状.
了解AsMaV种群结构对于管理整个欧洲的树健康至关重要.
AsMaV基因组包括五个负感单链RNA分子.

研究的目的:

调查阿斯马赛克相关病毒 (AsMaV) 的遗传多样性和种群参数.
确定用于研究AsMaV遗传多样性的代表性基因组区域.
分析AsMaV分离物的遗传关系和地理结构.

主要方法:

不同AsMaV基因组区域的放大和测序.
克隆和测序全长分离的AsMaV分离物.
开发RT-PCR-RFLP用于哈普洛型分析和大规模查.
病毒蛋白修饰的遗传学分析和生物信息学预测.

主要成果:

RNA3 (核蛋白) 是研究AsMaV遗传多样性的一个合适的代表.
RT-PCR-RFLP有效地确定了样本查高哈普洛型多样性地区.
遗传学分析揭示了来自瑞典和芬兰的AsMaV分离物的地理结构,可能是由于创始人效应.
AsMaV基因组被保存并处于净化选择阶段,与遥远的分离物和预测的翻译后修改位点的变异有关.

结论:

阿斯马赛克相关病毒 (AsMaV) 显示保存的基因组特征与可观测的地理结构.
RNA3 作为评估 AsMaV 遗传多样性的可靠标记.
RT-PCR-RFLP是有效查AsMaV种群的宝贵工具.

关键词:

这就是PCRRFLP.土耳其土耳其人土耳其人土耳其人土耳其人克隆,就是克隆人类.多样性的多样性多样性的多样性艾玛拉病毒 (emaravirus) 是一种病毒.森林的森林是森林的森林.全长全长的全长长的长度装饰性树木装饰性树木人类的基因组学.ssRNARNA 是一个

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