宿主衍生Sindbis病毒影响哺乳动物I型干扰素对感染的反应
John M Crawford1, Aaron M Buechlein2, Davis A Moline1
1Department of Biology, Indiana University, Bloomington, IN 47405, USA.
Viruses
|August 26, 2023
概括
来自蚊子的树冠病毒引发了更强的宿主反应,并在哺乳动物细胞中更快地关闭翻译. 这种快速翻译关闭可能会抵消干扰素反应,帮助病毒适应.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 阿尔博病毒适应在蚊子载体和哺乳动物宿主中复制.
- 蚊子衍生的病毒与哺乳动物衍生的病毒相比,表现出增强的哺乳动物细胞感染.
- 据假设,宿主特异性病毒适应会影响宿主反应.
研究的目的:
- 研究哺乳动物细胞对蚊子和哺乳动物衍生的辛迪斯病毒 (SINV) 的不同转录反应.
- 根据病毒源来阐明改变宿主细胞相互作用背后的机制.
主要方法:
- 对感染蚊子 (C7/10) 或哺乳动物 (BHK-21) 衍生的SINV.的人类胚胎293 (HEK-293) 细胞的RNA测序分析.
- 干扰素β (IFN-β) 和干扰素刺激基因 (ISG) 转录和翻译的量化.
- 测量化真核转化延长因子-2 (eEF2) 水平.
主要成果:
- 来自蚊子的SINV在HEK-293细胞中引起了更强大的转录反应.
- 尽管ISG转录量增加,但在感染了来自蚊子的SINV的细胞中,ISG翻译被抑制.
- 来自蚊子的SINV感染导致宿主细胞转化更快地关闭,与升高的p-eEF2相关.
结论:
- 病毒起源显著影响宿主细胞的转录和转化反应.
- 来自蚊子的SINV采用了诸如通过eEF2快速翻译关闭等机制,以潜在地逃避宿主抗病毒防御.
- 这些发现突出了哺乳动物宿主感染的树冠病毒适应策略.
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