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一种反转录循环介导的同热放大技术,用于检测番茄马赛克病毒,一种新兴的托巴莫病毒

Kan Kimura1, Akio Miyazaki1, Takumi Suzuki1

  • 1Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.

Viruses
|August 26, 2023
PubMed
概括

一种新的反转录循环介导同热放大 (RT-LAMP) 方法提供了快速和高度敏感的检测番茄斑马马赛克病毒 (ToMMV). 这种简单的技术有助于在番茄和胡中早期诊断ToMMV,这对于防止作物损失至关重要.

关键词:
土耳其人 RT-LAMP为了MMV,为了MMV.植物病毒 植物病毒这就是托巴莫病毒.牙器采样 取样 取样 取样

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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...