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银纳米颗粒被证明是有效的抗病毒在体外对抗三种高度致病性鱼类病毒

Andor Doszpoly¹, Mohamed Shaalan², Mansour El-Matbouli³

¹Veterinary Medical Research Institute, 21 Hungária krt., H-1143 Budapest, Hungary.

|August 26, 2023

概括

银纳米粒子 (AgNPs) 在对抗鱼类病毒如SVCV,ECV和IcHV-2方面表现有前途. 这项研究表明,AgNP在体外有效降低病毒载量,为水产养殖应用提供了潜力.

科学领域:

水生动物的健康状况.
纳米技术的应用.
在水产养殖中对病毒病的管理.

背景情况:

鱼类病毒,如鱼病毒的春季病毒病 (SVCV),欧洲鱼病毒 (ECV) 和Ictalurid疹病毒2 (IcHV-2) 在水产养殖业造成了巨大的经济损失.
有效的抗病毒策略对于可持续的水产养殖实践至关重要.
探索像银纳米粒子 (AgNPs) 这样的新型药物对于疾病控制至关重要.

研究的目的:

评估银纳米颗粒 (AgNPs) 对三种重要的鱼类病毒的体外疗效:SVCV,ECV和IcHV-2.
确定具有抗病毒活性,但不引起鱼细胞系中细胞毒性的AgNP的安全度.
在各种治疗条件和时间下调查AgNP的有效性.

主要方法:

在体外抗病毒试验中,使用银纳米粒子 (AgNPs) 对抗SVCV,ECV和IcHV-2进行了试验.
对EPC细胞进行了AgNP细胞毒性的评估,以确定安全度 (25 ng/mL).
在不同的治疗方案中应用AgNP:病毒前治疗,细胞前治疗和细胞后治疗,包括延迟治疗.

主要成果:

25 ng/mL AgNP 的安全度显著降低了所有三个测试病毒的病毒载量 (5-330×).
在所有测试的治疗条件中,AgNPs表现出有效性.
高度的AgNP甚至在延迟的细胞后处理实验中对ECV和IcHV-2有效.

结论:

银纳米颗粒在非细胞毒性度下对SVCV,ECV和IcHV-2具有显著的体外抗病毒活性.
在水产养殖中,AgNPs是开发新型抗病毒策略的有希望的候选者.
进一步研究AgNP的应用是有必要的,以支持鱼类健康和水产养殖的可持续性.

关键词:

欧洲鱼病毒伊克塔鲁里德疹病毒2 抗病毒效应抗病毒效应在体外测定试验.银纳米颗粒的使用方法鱼病毒的春季病毒病.

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