亚洲和地中海黄瓜种群之间的遗传变异 青虫产生的黄色病毒病毒
Parastoo Pouraziz1, Milad Yousefi1, Adyatma Irawan Santosa2
1Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan 45371-38111, Iran.
Viruses
|August 26, 2023
概括
在伊朗检测到黄瓜虫传染的黄虫病毒 (CABYV),显示出遗传多样性. 在亚洲和地中海两地都发现了伊朗CABYV分离物,这表明了显著的遗传分离.
科学领域:
- 植物病毒学 植物病毒学
- 分子遗传学分子遗传学
- 农业昆虫学 农业昆虫学
背景情况:
- 病毒性疾病在全球范围内导致黄瓜的大量产量损失.
- 黄瓜虫传染黄色病毒 (CABYV) 是影响黄瓜作物的主要病原体.
- 以前的研究还没有完全描述伊朗的CABYV多样性.
研究的目的:
- 来自伊朗的CABYV分离物的检测和遗传特征.
- 分析伊朗CABYV分离物的遗传多样性和种群结构.
- 了解该地区CABYV的进化关系和选择压力.
主要方法:
- 从伊朗的种植区 (2019-2022) 采集了200个有症状的虫样本.
- 用PCR放大P3和P4基因进行测序.
- 对完整的基因组和ORF进行遗传学,复合学和种群遗传学分析,包括公共数据库.
主要成果:
- 18个伊朗CABYV分离物被测序并存储在GenBank.
- ORF3/4序列的核酸标识范围从94.3%到99.5%.
- 伊朗的分离物聚集在亚洲和地中海分类中,具有很高的遗传分离 (FST>0.33) 和对基因和分类的负选择压力.
结论:
- 在伊朗,CABYV存在并具有遗传多样性,其分离物属于不同的亚洲和地中海血统.
- 克拉德之间有限的基因流动表明显著的遗传隔离.
- 了解CABYV的遗传多样性对于开发有效的虫疾病管理策略至关重要.
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