糖性乳酸生产支持实验动物的状态
Daria Skwarzynska1, Huayu Sun2, Izabela Kasprzak2
1Neuroscience Graduate Program, University of Virginia, Charlottesville, Virginia, 22908, USA.
Annals of clinical and translational neurology
|August 26, 2023
概括
通过酸盐抑制乳酸盐的产生,在小鼠中停止了状态 (SE),减少了大脑损伤和神经元死亡. 这表明向糖溶解是SE的有前途的治疗策略.
科学领域:
- 神经科学是一个神经科学.
- 生物化学 生物化学
- 细胞生物学 细胞生物学
背景情况:
- 状态 (SE) 是一种医疗紧急情况,需要立即治疗以防止脑损伤和死亡.
- 改变葡萄糖新陈代谢的性饮食对治疗耐火性SE.表示有前途.
- 乳酸盐在维持SE中的作用尚未完全理解,乳酸盐是糖解的产物.
研究的目的:
- 调查糖性乳酸生产在状态 (SE) 的进展中的作用.
- 评估抑制SE乳酸生产的治疗潜力.
主要方法:
- 使用生物传感器在期间体外葡萄糖和乳酸的体内测量.
- 施用牛酸盐,乳酸脱酶抑制剂,以阻断SE过程中酸盐转化为乳酸盐的转化.
- 评估发作的持续时间,严重程度,神经元损失 (免疫组织化学) 和神经元活动 (GCaMP7成像,SEPSC记录) 作为对oxamate的反应.
主要成果:
- 细胞外乳酸水平增加,而葡萄糖水平在和SE期间下降.
- 与对照组相比,胺酸治疗在小鼠中终止了SE,并显著减少了神经元损失.
- 在体外,oxamate降低了神经元刺激能力,并降低了刺激后突触电流的频率,表明了突触前效应.
结论:
- 糖溶性乳酸盐的生产对于维持SE至关重要.
- 用牛酸盐抑制乳酸盐的产生有效地终止SE,减少神经元死亡,减轻神经元刺激能力.
- 准糖解质乳酸盐的生产是管理状态的有希望的治疗策略.
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