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在新生猪组织中从4-基林合成甘氨酸

Shengdi Hu¹, Wenliang He¹, Fuller W Bazer¹

¹Department of Animal Science, Texas A&M University, College Station, TX 77843, USA.

Experimental biology and medicine (Maywood, N.J.)

|August 26, 2023

概括

小猪在他们的组织中从4-基烯中合成甘氨酸,以补偿母猪的低甘氨酸水平.

科学领域:

生物化学生化学
发展生物学发展生物学
动物营养动物营养

背景情况:

母猪奶为哺乳小猪提供不够的甘氨酸,只能满足它们20%的需求.
新生猪的内源性甘氨酸合成途径在很大程度上仍未被描述.

研究的目的:

为了研究新生猪小猪组织中从4-基林中内源合成甘氨酸.
确定关键的甘氨酸合成酶的活性和表达及其定位.

主要方法:

在不同年龄 (0,7,14,21天) 的小猪组织中,从4-基林合成甘氨酸的分析.
对糖氨酸合成酶 (OH-POX,POX,SHMT,TDH,AGT,HOA) 的酶活性和mRNA表达的测定.
酶活动的细胞特异局部化研究.

主要成果:

从4 - 西林合成甘氨酸观察到猪的肝脏和脏从出生,并在胰腺,小肠和骨肌肉14-21天的年龄.
关键酶 (OH-POX,AGT,HOA) 的酶活性和mRNA表达显示了与年龄相关的变化,在肠道和胰腺增加,而在肝脏和脏减少.
像普林氧化酶 (POX),氨酸甲基转移酶 (SHMT) 和三氨酸脱酶 (TDH) 这样的酶在各个组织和年龄段都存在.

结论:

4-氧作为猪内源性甘氨酸合成的关键前体.
糖氨酸合成途径的组织特异性和年龄依赖性调节在发展中小猪身上是显而易见的.
这些发现突出了新生猪食中糖氨酸缺乏症的补偿机制.

关键词:

氨基酸是氨基酸的组成部分.甘氨酸 (glycine) 是一种增长的增长增长的增长增长的增长.代谢过程中的代谢.新生儿们的新生儿营养营养营养营养

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