从冰淋工业废水中分离出来的Monascus ruber URM 8542优化L-glutaminase的生产
Sarah Signe do Nascimento1,2, Renan do Nascimento Barbosa3, Wellma de Oliveira Silva4
1Department of Mycology, Biosciences Center, Federal University of Pernambuco, Avenida Professor Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil. sarah.signe@ufpe.br.
World journal of microbiology & biotechnology
|August 26, 2023
概括
莫纳斯克 URM 8542 产生细胞外L-Glutaminase,一种具有潜在制药应用的酶. 确定了酶生产和稳定性的最佳条件,表明其有望进行进一步研究.
科学领域:
- 生物化学 生化学
- 酶学 是一种酶学.
- 微生物学 微生物学
背景情况:
- 氨酸酶是一种多用途的酶,在料和制药行业有应用.
- 它的抗病毒和抗质性质对药物开发特别感兴趣.
- 之前没有报告过Monascus ruber作为L-glutaminase的来源.
研究的目的:
- 为了识别和表征莫纳斯克虫URM 8542.2.的L-胺酶产量.
- 为了确定细胞外L-Glutaminase生产的最佳培养条件.
- 为了评估酶的稳定性和活性,以潜在的药理用途.
主要方法:
- 经典和分子分类学 (β-tubulin和calmodulin基因测序) 用于菌株识别.
- 浸泡发酵以优化L-谷氨胺酶的分泌.
- 完整的因数设计 (2^3) 评估L-氨酸度,温度和pH的影响.
- 在不同的pH值和温度下进行酶活性和稳定性测试.
主要成果:
- 蒙纳斯克木URM 8542被确定为L-Glutaminase的来源.
- 细胞外酶的产生是细胞内活动的11.4倍.
- 在L-Glutamine>1%的情况下,在pH值7.0和30°C时,产生最佳产量.
- 该酶在性pH (7.0-8.0) 和30°C至60°C的温度下表现出稳定性.
结论:
- 莫纳斯克草URM 8542是一种新型的细胞外L-氨酸酶的生产者.
- 鉴定出来的酶显示出适用于生物技术和潜在的药理学应用的有利特性.
- 进一步的研究是有必要的,以探索这种L-glutaminase的治疗潜力.
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