在压缩力后,PIEZO1促进牙周带细胞释放ATP
Seiwa Horie1,2, Chihiro Nakatomi1, Misa Ito-Sago1,2
1Division of Physiology, Kyushu Dental University, Fukuoka, Japan.
European journal of orthodontics
|August 26, 2023
概括
人体牙周带纤维细胞中机械敏感的PIEZO1通道被机械力激活,触发细胞外腺三酸盐 (ATP) 的释放. 这项研究证实了PIEZO1在这些细胞中机械刺激的ATP释放中的作用.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 矯正牙科 矯正牙科是一種矯正牙科.
背景情况:
- ортодонтические力量刺激细胞外腺三酸盐 (ATP) 在牙周带释放.
- 负责在牙周带细胞中释放ATP的特定机械敏感分子在很大程度上仍未得到证实.
- 皮埃佐通道是已知的机械传感器,但它们在人类牙周带纤维细胞 (HPdLF) 中机械刺激ATP释放的功能需要研究.
研究的目的:
- 研究机械敏感PIEZO通道在HPdLF中机械刺激ATP释放中的作用.
- 确认PIEZO通道在机械应力下ATP释放中的功能表达和参与.
主要方法:
- 使用定量PCR和免疫光检测用于检测HPdLF中的PIEZO通道表达.
- 一个体外重量负载细胞模型 (IVWLC) 已开发用于施加压缩力.
- 在使用PIEZO1激动剂 (Yoda1) 和机械压缩刺激后,测量ATP释放,使用或不使用特定通道抑制剂和PIEZO1基因沉默 (siRNA).
主要成果:
- 在HPdLF中,PIEZO1mRNA比PIEZO2mRNA丰富得多,PIEZO1蛋白表达得到证实.
- 应用Yoda1和压缩力剂量依赖性增加了细胞外ATP水平.
- 机械刺激诱导的ATP释放被PIEZO通道抑制 (GsMTx4),ATP释放途径抑制剂和PIEZO1siRNA显著减少.
结论:
- 位于HPdLF细胞膜上的PIEZO1通道通过机械压缩来激活.
- 激活PIEZO1,通过一种涉及细胞内和ATP透通道的机制,启动细胞外ATP释放.
- 这些发现阐明了在正治疗期间牙周带中发生机械传导的关键分子机制.
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