FANCM与MHF1-MHF2复合体相互作用,以限制大米化过程中的交叉频率
Yafei Li1, Yue Zhou1,2, Bingxin Wang1,2
1State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China.
The Plant journal : for cell and molecular biology
|August 26, 2023
概括
突变大米FANCM显著增加交叉频率,增强用于育种的遗传多样性. 这一发现加速了可取特征的整合,提高了作物改良效率.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 生殖生物学 生殖生物学
背景情况:
- 介质交叉 (COs) 在作物育种中对遗传多样性和特征集成至关重要.
- 保存的机制调节了CO的频率和分布,影响了繁殖效率.
研究的目的:
- 描述FANCM大米的特征,并阐明其在介质交叉控制中的作用.
- 通过增加COs来探索FANCM在提高米育种效率方面的潜力.
主要方法:
- 对米FANCM和各种ZMM突变 (hei10, ptd, shoc1, mer3, zip4, msh4, msh5, heip1) 的遗传分析.
- 研究了同源重组事件,HEI10标记和MUS81溶解酶依赖.
- 研究了与PAIR1,COM1,DMC1,HUS1,MEICA1和MHF复合体 (MHF1,MHF2) 的相互作用.
主要成果:
- 在不影响生育能力的情况下,FANCM突变增加了中的中介性CO频率.
- 粉丝突变中的额外COs是HEI10独立的,需要MUS81进行分辨.
- FANCM与PAIR1,COM1,DMC1,HUS1一起工作,并与MHF复合体相互作用.
- 破坏FANCM和MEICA1协同增加COs,但导致染色体异常.
- 切除MHF1或MHF2可以恢复zip4突变的双价形成.
结论:
- FANCM是大米中中性CO2的关键调节剂.
- 针对FANCM-MHF综合体提供了一种提高COs和加速米育种的战略.
- 通过FANCM-MHF复杂突变释放介质性COs通过促进理想的特征集成来提高繁殖效率.
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