核心基因组多部位序列类型分析芬兰泰洛瑞拉 (Taylorella equigenitalis) 隔离物
Kirsti Pelkola1, Sirpa Heinikainen2, Tarja Pohjanvirta2
1Animal Health Diagnostics Unit, Finnish Food Authority, Mustialankatu 3, Helsinki FI-00790, Finland.
Veterinary microbiology
|August 26, 2023
概括
这项研究使用全基因组测序对芬兰Taylorella equigenitalis (CEM) 隔离物进行基因型鉴定. 核心基因组MLST揭示了不同的遗传集群,突出了其未来传染性马甲状炎流行病学追踪的潜力.
科学领域:
- 兽医微生物学 兽医微生物学
- 基因组学就是基因组学.
- 流行病学 流行病学
背景情况:
- 传染性马甲状炎 (CEM) 是一种影响马匹的高度传染性疾病.
- 泰勒罗拉equigenitalis是导致CEM的细菌.
- 1992年,CEM首次在芬兰被发现,因此需要持续监测.
研究的目的:
- 为了基因鉴定芬兰Taylorella equigenitalis的分离物.
- 调查芬兰马群中CEM的流行病学模式.
- 评估核心基因组多位置序列类型化 (cgMLST) 对T. equigenitalis的实用性.
主要方法:
- 全基因组测序 (WGS) 在24匹马 (1992-2021) 的34个T. equigenitalis分离物上进行.
- 核心基因组多位置序列类型化 (cgMLST) 应用于1259个位置.
- 经典的MLST概况是从WGS数据中得出的,cgMLST分析是用于族系和集群评估的.
主要成果:
- 确定了三种新的MLST类型 (ST81,ST82,ST83) 和四种已知的类型 (ST16,ST17,ST50,ST63).
- cgMLST分析界定了五个不同的集群和三个单个集群.
- 每个分离物都具有独特的核心基因组序列类型,证明了cgMLST的高分辨能力.
结论:
- cgMLST提供了高分辨率的区分T.equigenitalis孤立,即使在相同的经典MLST类型.
- 这些发现有助于理解T. equigenitalis.的分类和时空分布.
- cgMLST显示出作为未来T. equigenitalis类型和流行病学研究的标准化方法的重大承诺.
关键词:
在 CEM CEM 中.传染性马匹甲状炎是一种传染性疾病.马类动物 马类动物格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁格尔丁在 MLST 上,我们可以看到 MLST.泰勒罗拉 (Taylorella equigenitalis) 是一种植物.cgMLST 的意思更多相关视频
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