基于TAMS 1基因的Theileria annulata分离物的综合遗传多样性和分子进化分析
Sanjhi Paliwal1, Daya Shanker2, Vikrant Sudan2
1College of Biotechnology, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura 281001, India.
Ticks and tick-borne diseases
|August 26, 2023
概括
使用TAMS 1基因的Theileria annulata的分子流行病学揭示了全球传播和最近的人口扩张. 这项研究突出了跨大陆相互连接的寄生虫菌株,表明了广泛的基因流和适应.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 分子流行病学分子流行病学
- 进化生物学 进化生物学
背景情况:
- Theileria annulata会引起热带乳牛病,这是一个重要的牛病.
- 遗传学特征对于理解寄生虫进化和控制至关重要.
- 甲素表面抗原1 (TAMS 1) 基因是分子研究的关键目标.
研究的目的:
- 来自印度的Theileria annulata分离物的遗传学特征进行.
- 在全球范围内使用TAMS 1基因分析T. annulata的遗传多样性和哈普洛型网络.
- 调查T. annulata.的进化历史和种群结构.
主要方法:
- 来自14个印度牛群的TAMS 1基因的放大和测序.
- 分析了来自16个国家的337个TAMS 1基因序列.
- 遗传学分析,哈普罗型网络构建和中立性测试 (塔吉马的D,Fu和李的F).
主要成果:
- 在14个印度隔离物中确定了5个单元型,在337个全球序列中确定了92个单元型.
- 哈普洛型14是最广泛的,在10个国家的46个分离物中发现.
- 在全球范围内观察到微不足道的遗传距离 (FST < 0.15) 和高基因流 (Nm > 1).
- 负的中性测试值表明最近的人口扩张是由积极选择驱动的.
结论:
- Theileria annulata 展现了广泛的全球分布,在不同地理区域有着密切联系的菌株.
- 遗传学分析支持缺乏明确定义的子组,符合高基因流动.
- 有证据表明,T. annulata最近的人口扩张,可能受到积极选择的影响.
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