相关实验视频

Updated: Jul 18, 2025

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Published on: April 19, 2017

探索尿液细胞外囊泡用于器官移植监测:使用免疫特异性标记物检测全移植功能障碍的综合研究

Anula Divyash Singh¹, Balakrishna Nagalla², Sreekanth Patnam¹

¹Apollo Hospitals Educational and Research Foundation (AHERF), Hyderabad, India; Department of Biomedical Engineering, Indian Institute of Technology Hyderabad (IITH), Kandi, Hyderabad, India.

Clinica chimica acta; international journal of clinical chemistry

|August 26, 2023

概括

尿液细胞外囊 mRNA 标记物显示出在移植后非侵入性检测全移植功能障碍 (AGD) 的前景. B细胞标记物表现出高灵敏度和特异性,为监测移植健康提供了一种新的策略.

科学领域:

腎臟病學 (nephrology) 是一種醫學.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

异位移植功能障碍 (AGD) 是固体器官移植 (SOT) 的常见并发症.
尿液细胞外囊泡 (UEVs) 提供了一个潜在的非侵入性生物标志物来源,用于检测AGD.
目前对AGD的诊断方法可能是侵入性的,可能会推迟治疗.

研究的目的:

评估UEV中T细胞和B细胞特异性mRNA标记物的诊断准确性,以检测AGD.
评估UEV-mRNA在接受移植患者的非侵入性监测方面的潜力.
识别特定的mRNA标记,表明T细胞介导和抗体介导的排斥.

主要方法:

从123名参与者中分离UEVS,包括健康对照组,功能移植接受者和活检证明的AGD患者.
使用RT-qPCR对T细胞和B细胞标记物mRNA表达的量化.
接收器操作特征 (ROC) 曲线分析以确定诊断性能.

主要成果:

在健康对照组和AGD患者之间观察到标记表达的显著差异.
B细胞标记物实现了高诊断准确度,具有100%的灵敏度和97.5%的特异性.
结合T细胞和B细胞标记物的联合特征显示了93.8%的灵敏度和95%的特异性,T细胞标记物的三元组提高了灵敏度.

结论:

针对T细胞和B细胞的UEV衍生的mRNA标记物对AGD具有显著的诊断潜力.
这种非侵入性方法为监测移植移植移植健康提供了一个有希望的策略.
进一步验证可能会导致SOT接受者的AGD临床管理得到改善.

关键词:

整体移植功能障碍 (AGD)通过B细胞介导的排斥 (ABMR)生物标志物的分析分析.固体器官移植 (SOT) 是一种实体器官移植.通过T细胞介导的排斥 (TCMR)尿液细胞外囊泡 (UEVs) 是一种

更多相关视频

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Published on: August 12, 2017

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

Published on: October 31, 2012

相关概念视频

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

Kidney Transplant I: Introduction

Kidney Transplant I: Introduction

A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...