形态上不同的氧酸盐纳米颗粒在多索菲拉菌中产生不同的基因毒性作用
Merve Güneş1, Burçin Yalçın1, Ayşen Yağmur Burgazlı1
1Department of Biology, Faculty of Sciences, Akdeniz University, Antalya, Turkey.
The Science of the total environment
|August 26, 2023
概括
合成酸纳米颗粒 (HAP-NP) 显示出基因毒性潜力,特别是圆形形式,影响DNA修复途径和细胞死亡. 环境暴露引发了人们的健康问题,需要仔细监测.
科学领域:
- 纳米材料的毒理学
- 环境健康 环境健康
- 遗传学 遗传学 是一个
背景情况:
- 酸纳米颗粒 (HAP-NP) 越来越多地用于骨科和环境修复.
- 对HAP-NP的广泛环境和人类暴露需要了解它们的潜在危害.
- 基因毒性和HAP-NP的细胞通路激活需要进行彻底的调查.
研究的目的:
- 为了研究Drosophila中圆形和棒形HAP-NP的基因毒性作用.
- 评估HAP-NPs.的细胞死亡途径 (酶活性) 的激活.
- 分析DNA修复基因的表达,以间接评估DNA损伤诱导.
主要方法:
- 用Drosophila翅膀点测试和彗星测试评估的基因毒性.
- 测量卡斯帕斯活性 (3/7,8,9),以检测亡.
- 定量PCR用于检查36个DNA修复基因的表达.
主要成果:
- 基因毒性通过翼点测定 (圆形HAP-NP) 和彗星测定 (两种形式) 证实.
- 增加的酶活动表明细胞死亡途径的激活.
- 在DNA修复途径中观察到差异性基因表达,不匹配修复基因增加,ATM,ATR和细胞循环途径基因减少.
结论:
- HAP-NP,特别是圆形形式,表现出具有基因毒性潜力.
- 观察到DNA修复基因表达的变化支持了基因毒性发现.
- 工程HAP-NP在环境中的存在需要对人类健康安全进行仔细评估.
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