Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Types of Membrane Protrusions01:28

Types of Membrane Protrusions

2.9K
The protrusion of the cell surface is an initial step for several cellular processes, including cell migration, phagocytosis, and neurite outgrowth. These membrane protrusions are a result of cytoskeletal rearrangement. The most  widely observed cell protrusions include lamellipodia, pseudopodia, filopodia, microvilli, invadopodia, and podosomes. These protrusions can be of two types — static or dynamic.
The microvilli, an example of stable protrusions, are finger-like projections...
2.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

R5 and R7 positions on fluoroquinolone scaffolds drive F-actin filament disruption.

Scientific reports·2026
Same author

The Yeast Parkinson's Disease Model Exhibits An Increase in Peroxisome Number Independent of the Division Proteins Vps1 and Dnm1.

Molecular neurobiology·2025
Same author

Yeast Dnm1<sup>G178R</sup> causes altered organelle dynamics and sheds light on the human DRP1<sup>G149R</sup> disease mechanism.

Mitochondrion·2025
Same author

DRP1: At the Crossroads of Dysregulated Mitochondrial Dynamics and Altered Cell Signaling in Cancer Cells.

ACS omega·2023
Same author

Insights into the role of the conserved GTPase domain residues T62 and S277 in yeast Dnm1.

International journal of biological macromolecules·2023
Same author

Biological activity of recombinant human PDX1 protein produced from Escherichia coli.

Journal of biochemical and molecular toxicology·2023

相关实验视频

Updated: Jul 18, 2025

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts &#8212; Implications for Neurodegenerative Disease Pathogenesis
09:09

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis

Published on: October 11, 2024

2.5K

注意差距:研究膜接触点的方法

Tanveera Rounaque Sarhadi1, Janhavee Shirish Panse1, Shirisha Nagotu1

  • 1Organelle Biology and Cellular Ageing Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.

Experimental cell research
|August 26, 2023
PubMed
概括

细胞器官通过膜接触点进行通信,这对于代谢物交换至关重要. 本综述讨论了研究这些动态细胞结构及其组成部分的关键方法.

科学领域:

  • 细胞生物学 细胞生物学
  • 生物化学 生物化学
  • 分子生物学分子生物学

背景情况:

  • 器官细胞对于细胞功能至关重要.
  • 膜接触部位 (MCS) 促进器官间的通信和代谢物交换.
  • 关于MCS的研究正在迅速扩大,确定了许多接触点和涉及的蛋白质.

研究的目的:

  • 审查和讨论研究膜接触点的基本方法.
  • 突出识别,测量和分析MCS的技术.
  • 提供MCS研究领域当前方法的概述.

主要方法:

  • 显微镜技术 (例如电子显微镜,超分辨率显微镜).
  • 生物化学试验 (例如,近距离结合试验,交叉连接).
  • 基因和蛋白质学方法用于识别MCS组件.
  • 活细胞成像用于MCS的动态分析.

主要成果:

  • 为了研究MCS存在各种方法,每个方法都有特定的优点和局限性.
  • 特定的蛋白质和脂质的识别,涉及到有机细胞膜的结合.
  • 在活细胞中量化MCS的大小,数量和持续时间.
关键词:
生物化生物化膜接触部位与膜的接触部位.显微镜的使用方法有机器人有机器人靠近的距离 靠近的距离

更多相关视频

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays
08:27

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

Published on: October 20, 2023

1.7K
Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
09:12

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

Published on: June 9, 2022

5.8K

相关实验视频

Last Updated: Jul 18, 2025

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts &#8212; Implications for Neurodegenerative Disease Pathogenesis
09:09

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis

Published on: October 11, 2024

2.5K
Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays
08:27

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

Published on: October 20, 2023

1.7K
Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
09:12

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

Published on: June 9, 2022

5.8K
  • 了解MCS在不同细胞过程中的功能相关性.

    • 结论:

    • 研究膜接触点需要多样化的方法方法.
    • 技术的进步显著提高了我们调查MCS的能力.
    • 进一步的方法开发将推动器官沟通和细胞功能方面的发现.