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聚基酸盐 (PHB) 积累由一个红树林隔离的蓝藻菌 Limnothrix planktonica使用水果废物

Prateeksha Mishra¹, Bhabatarini Panda²

¹Environmental Biology Research Laboratory, Department of Botany, Utkal University, Bhubaneswar, Odisha, India.

International journal of biological macromolecules

|August 26, 2023

概括

使用蓝色细菌Limnothrix planktonica的水果废弃物回收增强了聚基酸盐 (PHB) 的生产. 香皮产生了最高的PHB,缺乏进一步增加了5倍的积累.

科学领域:

生物技术是生物技术.
环境科学环境科学
微生物学微生物学

背景情况:

蓝菌聚酸 (PHB) 为细菌生产提供了一种经济高效且可持续的替代品.
果物废弃物的利用与循环经济原则和环境可持续性保持一致.
Limnothrix planktonica是PHB积累的一个有前途的蓝藻.

研究的目的:

研究各种水果废物的潜力,作为Limnothrix planktonica中PHB积累的碳来源.
优化培养条件,包括媒介变化和剥皮预处理,以提高PHB产量.
为了比较使用水果废弃物的PHB生产与商业碳来源和光自otrophic条件.

主要方法:

使用香,子,豆,子和西瓜皮作为碳来源种植Limnothrix planktonica.
修改ASN-III培养基和对果皮进行酸预处理.
在不同的条件下进行化,包括 (NP) 缺乏,以最大限度地提高PHB积累.
PHB分子重量的表征.

主要成果:

香皮显示PHB积累率最高 (25.73 mg/L),超过商业糖 (果糖,葡萄糖).
与香皮补充的NP缺乏介质导致PHB (39.39 mg/L) 与光自营生长相比增加了5倍.
从水果废弃物中获得的PHB的分子量高于在光自otrophic条件下产生的PHB.

结论:

水果废物,特别是香皮,是菌PHB生产的有效和可持续基质.
优化条件,如NP缺陷,显著提高PHB产量和质量.
对参数优化和PHB表征的进一步研究可以释放其工业潜力.

关键词:

香皮是一种香皮.林诺特里克斯浮游生物 Limnothrix PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB PHB

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