多化,EZH2上调和转化在受细胞巨乳病毒感染的人类卵巢上皮细胞中
Ranim El Baba1, Sandy Haidar Ahmad1, Franck Monnien2
1Department of Pathogens & Inflammation-EPILAB Laboratory EA4266, University of Franche-Comté, Besançon, France.
Oncogene
|August 26, 2023
概括
人类细胞巨乳病毒 (HCMV) 转化卵巢细胞,为上皮卵巢癌 (OC) 创造了一个模型. 这种感染升级EZH2,促进癌症干细胞特征和多化巨型癌细胞 (PGCCs).
科学领域:
- 在瘤学瘤学.
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 人类细胞巨核病毒 (HCMV) 与上皮卵巢癌 (OC) 有关.
- 具有干状特征的多化巨型癌细胞 (PGCC) 在高度血清性卵巢癌 (HGSOC) 中被发现.
- EZH2与OC进展和瘤等级有关.
研究的目的:
- 研究HCMV作为人类卵巢上皮细胞 (OEC) 中的重编程载体的作用.
- 建立一个由HCMV诱导的上皮卵巢癌模型.
- 探索EZH2在HCMV介导的OEC转化中的作用.
主要方法:
- 用高风险HCMV菌株 (DB和BL) 感染OECs.
- 用EZH2抑制剂治疗以评估其作用.
- 从HGSOC活检中分离和描述HCMV菌株.
- 产生和分析"CMV转化卵巢细胞" (CTO).
主要成果:
- HCMV感染将OEC转化为CTO细胞,显示出增殖潜力和PGCC生成.
- 在OEC中,HCMV感染导致EZH2上调.
- 抑制EZH2降低了CTO细胞中的增殖和PGCC生成.
- 在HGSOC活检中,增加的EZH2表达和HCMV存在存在相关性.
结论:
- 高风险的HCMV菌株可以转化OEC,支持HCMV诱导的卵巢癌模型.
- EZH2在HCMV介导的卵巢癌的发展中起着显著的瘤发生作用.
- 向HCMV和EZH2可能为卵巢癌提供新的治疗策略.
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