用后列注入分析HPLC的验证作为一种快速准确量化放射化学产量的方法
Swen Humpert1, Chris Hoffmann2, Felix Neumaier2
1Forschungszentrum Jülich GmbH, Institute of Neurosciences and Medicine, Nuclear Chemistry (INM-5), Wilhelm-Johnen-Str., 52428 Jülich, Germany.
概括
精确的放射化学产量 (RCY) 评估对于优化放射标记反应至关重要. 一种用于高性能液态染色学 (HPLC) 分析的新型后列注射方法可靠地确定放射化学转换 (RCC) 并预测孤立的RCY,提高准确性和效率.
科学领域:
- 放射化学和核医学 放射化学和核医学
- 分析化学 分析化学
- 染色图学技术 染色图学技术
背景情况:
- 精确的放射化学产量 (RCY) 对于优化放射标记反应至关重要.
- 放射化学转换 (RCC) 通常使用HPLC进行估计,但列上的活动保留可能会导致不准确.
- 现有的RCC测定方法可能会高估实际产量,因为放射性恢复不完全.
研究的目的:
- 在HPLC分析中验证一种实用的后列注射方法,以准确确定RCC.
- 评估该方法在放射标记反应中预测孤立RCY的可靠性.
- 为了比较后列注射的准确性和效率与传统的HPLC分析.
主要方法:
- 开发并实施了与HPLC集成的柱后注入系统.
- 使用各种C18HPLC列和移动相分析了2-[18F]化 ([18F]FPy) 和[18F]CPFPX的模拟反应混合物.
- 通过总结的峰值面积与后列注射相比,并与孤立的RCY计算分析RCY.
主要成果:
- 在某些条件下,传统的总和峰值面积方法高估了RCC高达50%.
- 在所有测试的染色学条件下,后列注射提供了精确的RCC确定.
- 来自后列注射RCCs的分析RCYs与孤立的RCYs显示出很好的一致性 (<3%的偏差).
结论:
- 使用后列注射的HPLC分析可使可靠的,独立于条件的RCC确定.
- 这种方法与活动平衡相结合,可以快速准确地预测孤立的RCYs.
- 经过验证的方法增强了放射性标记反应的优化,并改善了整体放射性药物开发.
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