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在由大肠杆菌 (Escherichia coli) 产生的依赖NAD的酵素MaeA中的酶活性

Aqeel Rana Afzal¹, Jinyoung Jeon¹, Che-Hun Jung²

¹Department of Medical Science, Chonam National University, Gwangju, 61186, South Korea.

Biochemical and biophysical research communications

|August 27, 2023

概括

大肠杆菌的酶A (MaeA) 显示出烟酶活性,将烟酸转化为酸盐,然后是酸盐. 这种在人类ME2中也观察到的双重功能,表明依赖NAD的酶具有保留的酶能力.

科学领域:

生物化学生物化学
酶学是一种酶学.
分子生物学分子生物学

背景情况:

已知依赖NAD的酶可催化酸盐转化为酸盐,从而将NAD减少为NADH.
研究了大肠杆菌 (Escherichia coli) 酶A (MaeA) 的酶谱,以寻找新型活动.

研究的目的:

调查MaeA.的基质特异性和潜在的双重酶活性.
为了确定 fumarate 是否可以作为由 MaeA 减少 NAD 的基质.
探索不同物种中烟酶和酶活动的保存.

主要方法:

用不同度的烟酸盐和酸盐进行了酶动力学测试.
通过光谱光度测量监测NAD减少到NADH.
对人类ME2和大肠杆菌MaeB进行了比较分析.

主要成果:

在烟酸盐的存在下,MaeA催化了NAD降解为NADH,表明了烟酶活性.
烟酸的K0.5值被确定为13mM.
发现,当酸是基质时,酸会抑制MaeA的酶活性.
人类ME2表现出类似的双重活性,而大肠杆菌MaeB没有表现出烟酶活性.

结论:

大肠杆菌 (Escherichia coli) MaeA具有双重的酶功能,同时作为一个酶和一个烟酶.
这种双重活性,即将烟酸转化为酸盐,然后转化为酸盐,似乎在人类ME2中保持着.
这种双重功能的特异性在NADP依赖的酶MaeB.中缺乏类似活动时得到了强调.

关键词:

ME2 ME2 的意思是什么?美国美国美国美国美国美国美联储 MaeB MaeB 马利克酶马利克酶是什么?代谢途径的代谢途径

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