纸质分析装置与菌体尾部纤维蛋白集成,用于细菌检测和抗微生物敏感性测试
Yu Zhang1, Zhongjie Zhu2, Yuchan Ma1
1The State Key Lab of Resource Insects, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China.
Biosensors & bioelectronics
|August 27, 2023
概括
一种基于纸张的新型设备提供了快速,灵敏和负担得起的光检测和针对Pseudomonas aeruginosa感染的抗菌感应性测试. 这种方法有助于临床诊断,特别是在资源有限的环境中.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 微生物学 微生物学
背景情况:
- 抗菌素耐药性需要快速且负担得起的诊断工具.
- 目前用于细菌检测和敏感性测试的方法可能是缓慢而昂贵的.
- Pseudomonas aeruginosa 是一个重要的机会性病原体.
研究的目的:
- 开发一种基于纸张的分析装置 (PAD) 用于光检测Pseudomonas aeruginosa.
- 为了使P. aeruginosa.能够进行快速的抗微生物敏感性测试 (AST).
- 为资源有限的环境提供一个负担得起的诊断平台.
主要方法:
- 使用液体印刷制造96微区PAD的制造.
- 斯特雷普塔维丁的固定和生物化细菌尾纤维蛋白 (TFP) 的结合.
- 使用TFP作为捕获剂,并使用标记为fluorescein isothiocyanate (FITC) 的抗微生物作为光检测的信号探针.
主要成果:
- 获得了P. aeruginosa量化1.0 x 10^3到1.0 x 10^7 CFU/mL的线性范围.
- 确立了137 CFU/mL的检测极限.
- 与多种抗生素的传统稀释相比,证明了一致的AST结果.
结论:
- 开发的PAD是有效的敏感和快速光检测P. aeruginosa.
- PAD成功进行了针对P. aeruginosa的抗微生物敏感性测试.
- 这项技术在资源有限的环境中为细菌感染诊断提供了有希望的,负担得起的解决方案.
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