在生长过程中减少口会通过影响三角质,调节Wnt信号通路和ARHGAP33分子传输来抑制认知功能
Eri Misawa-Omori1, Hidemasa Okihara1, Takuya Ogawa1
1Department of Orthodontic Science, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan.
Neuropeptides
|August 27, 2023
概括
在发育过程中减少会通过改变大脑衍生神经营养因子 (BDNF) 和其受体TrkB信号通路在海马和三角质中,损害小鼠的记忆和学习.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 大脑衍生神经营养因子 (BDNF) 和它的受体氨酸激酶B (TrkB) 对海马发育,记忆和学习至关重要.
- 影响海马BDNF和TrkB表达,刺激减少可能导致TrkB降低和BDNF水平改变.
- Wnt家族成员3A (Wnt3a) 和Rho GTPase激活蛋白33 (ARHGAP33) 与BDNF/TrkB信号传递和细胞内TrkB传输有关.
研究的目的:
- 为了研究减少小鼠刺激会损害记忆和学习的假设.
- 为了检查减少口对海马体Wnt3a和ARHGAP33表达的影响.
- 阐明与改变的输入相关的潜在记忆缺陷的分子机制.
主要方法:
- 给小鼠食粉状饮食,直到14周大,以模拟减少的刺激.
- 实时聚合酶连锁反应 (PCR) 用于分析海马体中的BDNF和TrkBmRNA表达.
- 西方涂抹评估了Wnt3a和ARHGAP33蛋白质水平,而免疫组织化学评估了海马体和三角管 (TG) 中的蛋白质表达和神经元形态.
主要成果:
- 减少的刺激显著改变了海马体中BDNF,Wnt3a,ARHGAP33和TrkB蛋白的表达.
- 减少的小鼠表现出记忆和学习能力受损.
- 实验组显示TG神经元数量显著减少,细胞间空间面积增加.
结论:
- 在发育过程中减少的刺激会对记忆和学习功能产生负面影响.
- 这些认知缺陷与海马体内关键分子媒介体的表达改变有关,包括BDNF,Wnt3a和ARHGAP33.
- 通过口输入对海马体和三腺中分子传播的调节在认知功能中起着至关重要的作用.
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