从大米水解盐中生产聚氨酸酸盐:洞察饥荒动态和微生物社区转移的洞察力
Raj Morya1, Fanirintsoa Herivonona Andrianantenaina1, Ashutosh Kumar Pandey1
1Department of Civil and Environmental Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
Chemosphere
|August 27, 2023
概括
废弃物活性污泥 (WAS) 可以通过盛宴-饥荒周期产生多基酸盐 (PHA). 这项研究确定了关键细菌,并实现了3.5g/L的Poly ((hydroxybutyrate-co-hydroxyvalerate) (PHBV).
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 环境科学 环境科学
背景情况:
- 活性污泥含有多种能够合成生物聚合物的微生物群落.
- 废弃活性污泥 (WAS) 是生产有价值化学品的可持续来源.
- 聚酸酸盐 (PHA) 是一种可生物降解的聚合物,具有多种应用.
研究的目的:
- 通过WAS研究反复的盛宴和饥荒周期对PHA生产的影响.
- 分析PHA积累期间的微生物种群动态.
- 描述产生的PHA的结构和组成.
主要方法:
- 使用废弃活性污泥 (WAS) 作为PHA生产的注射剂.
- 用米水解剂作为碳基质.
- 进行了16S rRNA基因测序,用于微生物社区分析.
- 使用富里埃变换红外光谱 (FTIR) 和核磁共振 (NMR) 进行了PHA的特征.
主要成果:
- 达到PHA度为3.5 ± 0.2 g/L. 这一结果是:
- 确定了科里尼菌群 (40%),百日菌群 (23%) 和伪菌群 (5%) 作为占主导地位的PHA生产者.
- 百花科和伪虫在盛宴和饥荒阶段表现出弹性.
- 生产的PHA被确定为聚乙酸-联合乙酸 (PHBV).
结论:
- 重复的盛宴和饥荒周期有效地提高了WAS的PHA产量.
- 特定的细菌群体,包括Bacillaceae和Pseudomonas,在动态条件下对PHA合成至关重要.
- 米水解剂是PHBV生产的可行基质.
- 该研究表明了WAS在可持续生物聚合物制造方面的潜力.
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