使用人类肠道微生物生态系统模拟器 (SHIME) 对肠道微生物群的二二乙暴露的影响
Yasong Luo1, Mai Zhang2, Shuyang Huang2
1NMPA Key Laboratory for Safety Evaluation of Cosmetics, Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Environmental Health, School of Public Health, Southern Medical University, Guangzhou, 510515, China; Guoke (Foshan) Testing and Certification Co., Ltd, Foshan, 528299, China.
Chemosphere
|August 27, 2023
概括
暴露于Tris (2-chloroethyl) 酸盐 (TCEP) 会改变人类肠道微生物组的结构和功能. 高剂量的TCEP增加了肠道细菌的多样性,并改变了微生物组成,影响了短链脂肪酸的产生.
科学领域:
- 环境健康 环境健康
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 酸二二 (TCEP) 是一种广泛使用的化学物质,对健康问题越来越关注.
- 关于TCEP对人类肠道微生物组结构和代谢功能的影响的研究有限.
- 了解这些影响对于评估TCEP的整体健康风险至关重要.
研究的目的:
- 调查TCEP暴露对人类肠道细菌社区和结构的影响.
- 分析TCEP对肠道微生物群的代谢功能的影响,特别是短链脂肪酸 (SCFA) 生产.
主要方法:
- 使用了人类肠道微生物生态系统模拟器 (SHIME) 模型.
- 采用16S rRNA基因的高通量测序来分析肠道微生物社区的组成.
- 在不同结肠部分测量了短链脂肪酸 (SCFA) 的度.
主要成果:
- 高剂量的TCEP暴露增加了肠道微生物群的α多样性 (香农和辛普森指数).
- 暴露于TCEP改变了微生物的组成,增加了Firmicutes,并减少了Bacteroidetes在类层面.
- 观察到SCFA度的显著变化,乙酸盐和酸盐降低,酸盐增加.
结论:
- 暴露于TCEP显著改变了人类肠道微生物群体的组成和代谢功能.
- 像Roseburia和Lachnospira这样的特定细菌属显示与TCEP暴露剂量和SCFA产生的相关性.
- 这些发现突出了TCEP暴露通过肠道微生物组破坏的潜在负面健康影响,这引起了公众的关注.
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