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在Pichia pastoris中表达的抗菌PMAP-37 (((F34-R) 对病原细菌有效,并保存梅子

Chunming Dong¹, Lijun Xu², Weitao Lu²

¹College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin, 300457, China. mingchundongjy@tust.edu.cn.

Microbial cell factories

|August 27, 2023

概括

研究人员使用Pichia pastoris开发了一种新型抗微生物,PMAP-37(F34-R),使用Pichia pastoris. 这种显示出广泛的抗菌活性,并有效地保存梅子,为传统抗生素提供了潜在的替代品.

科学领域:

生物化学生物化学
微生物学微生物学
食品科学食品科学食品科学

背景情况:

由于耐药性,越来越需要替代传统抗生素的替代品.
抗微生物 (AMP) 提供抗菌活性,降低了耐药性风险.
猪骨髓状抗菌37 (PMAP-37) 和它的模拟物PMAP-37 (F34-R) 是一个有前途的产品.

研究的目的:

在Pichia pastoris.中,PMAP-37 (((F34-R) 的重组表达.
研究抗菌性质和作用机制.
评估梅子的防腐剂潜力.

主要方法:

对于重组PMAP-37 (F34-R) 表达的等离子体构造.
引入Pichia pastoris用于酸生产.
确定最小抑制度 (MIC) 和细胞膜完整性的破坏.
血液溶解测定哺乳动物细胞安全性.
作为梅子防腐剂的应用试验.

主要成果:

获得的高活性重组PMAP-37 (((F34-R).
广泛抑制格拉姆阳性和格拉姆阴性细菌 (MIC:0.12-0.24μg/mL).
PMAP-37 (F34-R) 破坏了细胞膜的完整性,导致溶解.
在有效度下,低血解率 (<1.5%).
梅子腐烂和体重减轻显著减少;呼吸延迟.

结论:

再组合的Pichia pastoris菌株成功地产生了活性PMAP-37 (F34-R).
PMAP-37 (F34-R) 显示出强大的抗菌活性和膜破坏机制.
果的有效防腐剂,延长了保质期.
开发新的,安全和有效的食品防腐剂的潜力.

关键词:

抗菌机制是一种抗菌机制.抗微生物是一种抗微生物.保护保护保护

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