对来自Ocimum basilicum的主要生物活性化合物的分析,以检测它们的抗微生物和抗氧化活性
Arockia Doss Susai Backiam1, Senbagam Duraisamy2, Palaniyandi Karuppaiya3
1Department of Microbiology, Vivekanandha College of Arts and Science for Women (Autonomous), Tiruchengode, Tamil Nadu, India.
Biotechnology and applied biochemistry
|August 28, 2023
概括
巴西里叶提取物显示出强大的抗微生物和抗氧化特性,可以对抗抗康氏素耐药性肠球菌 (VRE). 乙醇提取物表现出最强烈的活性,通过破坏细胞膜来抑制VRE和其他微生物.
科学领域:
- 植物化学 植物化学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 对植物生物活性剂的兴趣日益增长,用于治疗耐万科米肠球菌 (VRE) 感染.
- 种在传统医学和食品中具有悠久的历史,但它们的生物活性化合物尚未得到充分探索.
研究的目的:
- 为了评估玄米叶提取物的抗菌和抗氧化活性.
- 研究白提取物对VRE的植物化学成分和作用机制.
主要方法:
- 使用乙醇,甲醇和水提取玄米叶.
- 植物化学查,确定总和黄含量.
- 对VRE和MTCC菌株进行抗菌活性测试 (MIC确定).
- 使用UV-Vis光谱仪和扫描电子显微镜的作用研究的机制.
- 气色谱-质谱 (GC-MS) 分析乙醇提取物的分析.
主要成果:
- 乙醇和甲醇提取物含有广泛的植物化学物质,而水性提取物缺乏素,类固醇和类物质.
- 在乙醇中观察到更高的和黄酸含量,其次是甲醇和水性提取物.
- 乙醇和甲醇提取物对VRE和MTCC菌株表现出显著的抗菌活性,最小抑制度 (MIC) 范围为14-20 mg/mL.
- 金黄色葡萄球菌对乙醇提取物 (8毫克/毫升) 特别敏感.
- 抗菌活性归因于细胞膜损伤和完整性丧失.
- 所有的提取物都表现出显著的抗氧化活性.
- 在GC-MS分析中,乙醇提取物中发现了19种化合物,具有潜在的治疗和食品应用.
结论:
- 叶提取物,特别是乙醇和甲醇提取物,具有针对VRE的显著抗菌和抗氧化潜力.
- 抗菌机制涉及破坏细菌细胞膜的完整性.
- 巴西里叶的植物化学物质代表了开发新型抗耐药细菌菌株的有希望的来源.
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