在植物START域中基于腔结构调制连接体结合道
Sanjeet Kumar Mahtha1, Kamlesh Kumari1, Vineet Gaur1
1National Institute of Plant Genome Research, New Delhi 110067, India.
Computational and structural biotechnology journal
|August 28, 2023
概括
植物类固醇性急性调节蛋白 (StAR) 相关的脂质转移 (START) 域显示结构变异. 这些腔变化减少了连接体结合道,导致它们与哺乳动物对应物之间的功能分歧.
科学领域:
- 结构生物学是结构生物学.
- 进化生物学是进化的生物学.
- 植物生物化学 植物生物化学
背景情况:
- 类固醇急性调节蛋白 (StAR) 相关的脂质转移 (START) 域是一个保存的脂质转移蛋白超级家族.
- 植物START领域已经得到了显著的扩展,但人们对其了解很少.
- 腔结构变异是蛋白质进化和功能的关键.
研究的目的:
- 探索植物START蛋白质进化和功能多样性中的腔体结构调制.
- 为了比较植物和哺乳动物的START域结构和结合偏好.
- 为了弥合植物START领域功能的知识差距.
主要方法:
- 深度学习方法用于生成植物START域模型.
- 米START家族的表面可访问性研究和结构调查.
- 结合腔的划定和体积测量,与哺乳动物的START域进行比较.
主要成果:
- 验证了28个大米START领域模型,识别植物中独特的腔内残留物 (CLRs).
- 与哺乳动物相比,由于特定的CLRs,植物START域表现出较小的联结道.
- 第四型HD-Zip家族成员表现出被消灭的腔,表明转向DNA结合.
结论:
- 植物START域保留了祖先的特征,但显示出显著的结构和功能差异.
- 腔结构调制是植物START蛋白中功能转移的关键机制.
- 这项研究量化了植物START结构分歧,为它们的进化轨迹提供了洞察力.
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