线粒体DNA突变可以影响人类马赛克胚胎的植入后发育
Akifumi Ijuin1,2, Hiroe Ueno1, Tomonari Hayama1,3
1Reproduction Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.
Frontiers in cell and developmental biology
|August 28, 2023
概括
人类的马赛克胚胎发育受到线粒体功能的影响. 减少线粒体DNA突变与胚胎发育的改善以及植入后形细胞的减少相关.
科学领域:
- 生殖生物学 生殖生物学
- 遗传学 是一个遗传学.
- 线粒体生物学 线粒体生物学
背景情况:
- 包含euploid和aneuploid细胞的马赛克胚胎可以导致健康的出生.
- 在人类中,马赛克胚胎中无体积的自我纠正还没有完全被理解.
- 线粒体功能对胚胎发育至关重要,可能在减少无体积症方面发挥作用.
研究的目的:
- 研究线粒体功能在人类植入后胚胎发育中的作用.
- 为了确定线粒体DNA (mtDNA) 突变是否会影响马赛克胚胎中形细胞的减少.
- 探索选择可行的马赛克胚胎进行移植的新方法.
主要方法:
- 人类胚胎 (n=27) 在初始活检后在体外培养,以模拟植入后的发育.
- 型定型,整个线粒体DNA测序和mtDNA复制数测定在培养前和培养后的样本上进行.
- 分析的重点在于在体外培养过程中 ploidy 和 mtDNA 特征的变化.
主要成果:
- 在体外培养后观察到,欧胚胎的比例显著增加,马赛克胚胎的数量减少.
- 欧体和可活的培养后胚胎显著减少了线粒体DNA突变.
- 两组之间在线粒体DNA拷贝数没有发现显著差异.
结论:
- 人类胚胎在植入后的发育过程中,形细胞的数量会减少.
- 线粒体DNA突变可能会损害线粒体功能,影响euploid和aneuploid胚胎的发育.
- 分析整个线粒体DNA突变负担可能为选择马赛克胚胎进行移植提供了一种新方法.
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