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对lncRNAs和circRNAs的全面识别和表征揭示了大米中潜在的棕色植物响应的ceRNA网络

Yan Wu^1,2, Wenjun Zha^1,2, Dongfeng Qiu¹

¹Key Laboratory of Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan, China.

Frontiers in plant science

|August 28, 2023

概括

这项研究确定了响应棕色虫 (BPH) 侵袭的水植物中的长非编码RNA (lncRNA) 和圆形RNA (circRNA). 这些非编码RNA在防治这种破坏性害虫的水防御网络中发挥着重要作用.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
RNA生物学的RNA生物学

背景情况:

棕色虫 (BPH) 是一个主要的水害虫.
非编码RNAs调节生物过程,但它们在米-BPH相互作用中的作用尚不清楚.

研究的目的:

在BPH感染下的水植物中识别和表征长非编码RNA (lncRNAs) 和圆形RNA (circRNAs).
为了研究这些RNA在米BPH耐药性中的潜在功能.

主要方法:

使用深度RNA测序对lncRNAs和circRNAs进行全基因组分析.
在BPH养之前和之后,对抗性和易受性大米线的差异表达分析.
使用生物信息学工具 (GO,KEGG) 预测lncRNA和circRNA作为具有竞争力的内源RNA (ceRNA) 的功能.
使用双露西法酶记者分析验证miRNA-lncRNA相互作用.

主要成果:

总共有310个差异表达的lncRNA和129个差异表达的circRNA被确定.
预计39个lncRNA和21个circRNA与133个常见的miRNA相互作用,可能与834个mRNA竞争.
这些相互作用表明细胞壁生物发生,生长,细胞过程和应激反应的参与.

结论:

这项研究提供了在米-BPH相互作用中的lncRNAs和circRNAs的全面识别和功能特征.
这些发现揭示了一个潜在的ceRNA网络,有助于大米对BPH的耐药性.
这项研究为了解植物害虫相互作用中的非编码RNA功能奠定了基础,并增强了对大米BPH耐药性机制的了解.

关键词:

棕色的植物跳圆形的RNARNA是一个圆形的RNA.具有竞争力的内源性RNA.长非编码RNA是什么意思米米饭米饭米饭米饭.

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