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甲基伊索提亚林诱导人体角质细胞中ER压力诱导的压力颗粒形成

Da-Min Jung¹, Kee K Kim¹, Eun-Mi Kim²

¹Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon, Republic of Korea.

Animal cells and systems

|August 28, 2023

概括

作为消毒剂的甲基异索利 (CMIT) 通过触发压力颗粒和内分泌网膜压力来损害皮肤细胞. 即使短暂的暴露也会损害人类的角质细胞,表明潜在的皮肤毒性.

科学领域:

皮肤病学皮肤病学
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

甲基异索利 (CMIT) 是一种常见的消毒剂,已知具有呼吸道毒性.
在CMIT的皮肤效应仍然在很大程度上未被描述.
研究CMIT对皮肤细胞的影响对于了解其完整的毒理学特征至关重要.

研究的目的:

研究CMIT对人类角质细胞的细胞毒性作用.
检查应力颗粒 (SG) 形成作为CMIT诱导的细胞损伤的标志物.
阐明CMIT对皮肤细胞影响的分子途径.

主要方法:

在CMIT暴露后评估了人类角质细胞细胞活力.
使用免疫光显微镜检测压力颗粒形成.
进行了免疫块分析,以检查下游的信号通路,包括eIF2α酸化.

主要成果:

暴露于CMIT诱导了人类角质细胞中显著的压力颗粒形成.
观察到真核启动因子-2α (eIF2α) 和蛋白激酶R型内细胞网膜激酶的酸化.
细胞活力测试显示IC50值表明CMIT暴露后的毒性.

结论:

暴露于CMIT会诱导人体角质细胞中的压力颗粒形成,这表明对皮肤的细胞毒性作用.
细胞内膜网膜 (ER) 的压力与CMIT诱导的角质细胞损伤有关.
短暂接触CMIT可以对皮肤产生有害影响,强调需要谨慎.

关键词:

甲基伊索提阿索利诺细胞内膜网膜压力-压力氨酸细胞 (keratinocyte) 是一种细胞.蛋白质激酶是R型的内oplasmic网膜激酶.压力颗粒是一种压力颗粒.

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