通过共同表达网络分析引入与直肠癌对化疗反应相关的新型关键基因和转录因子
Saeid Afshar1, Tapak Leili2, Payam Amini3
1Cancer Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Heliyon
|August 28, 2023
概括
预测结直肠癌对手术前化学放射治疗的反应至关重要. 这项研究确定了四个关键基因 (ZEB2,JAM2,NDN,PPAP2A) 和七个转录因子作为直肠癌患者治疗反应的潜在生物标志物.
科学领域:
- 在瘤学瘤学.
- 基因组学就是基因组学.
- 系统生物学 系统生物学
背景情况:
- 术前放射化疗是局部晚期直肠癌的关键治疗方法.
- 患者对这种治疗的反应有显著的变化.
- 识别预测生物标志物对于个性化治疗策略至关重要.
研究的目的:
- 确定预测结直肠癌 (CRC) 对术前放射治疗反应的新生物标志物.
- 用系统生物学方法来发现生物标志物.
主要方法:
- 使用权重基因共同表达网络分析 (WGCNA) 来构建基因共同表达网络.
- 评估了模块-特征关系,以确定与辐射反应相关的网络.
- 使用生物信息学工具识别了重要的模块内的枢纽基因和相关的转录因子.
主要成果:
- 一个显著的共同表达模块 (113个节点,6066个边缘) 与辐射效应有很强的相关性 (相关性=0.85,p=6e-7).
- 在这个模块中,确定了四个枢纽基因 (ZEB2,JAM2,NDN,PPAP2A).
- 还确定了可能调节这些枢纽基因的七个关键转录因子 (KLF4,SUZ12,TCF4,NANOG,POU5F1,SOX2,SMARCA4).
结论:
- ZEB2,JAM2,NDN和PPAP2A及其相关的转录因子可以作为结直肠瘤对化学放射治疗反应的预测生物标志物.
- 这些发现有助于理解治疗变异性,并开发直肠癌向治疗的向疗法.
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