进一步证实了一个新兴的无刺蜂-酵母共生体
Gabriela Toninato de Paula1, Weilan Gomes da Paixão Melo1,2, Ivan de Castro3
1Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Frontiers in microbiology
|August 28, 2023
概括
这项研究揭示了无刺蜂和Zygosaccharomyces酵母之间的共生关系. 这些酵母提供了对蜜蜂转型至关重要的厄戈斯特醇,这表明社会昆虫中普遍存在互惠主义.
科学领域:
- 微生物学 微生物学
- 昆虫学 昆虫学是一门学科.
- 同生相互作用的交互.
背景情况:
- 社会昆虫依赖微生物共生体来生存和发育.
- 没有刺的蜜蜂Scaptotrigona depilis利用Zygosaccharomyces酵母来产生厄戈斯特醇,这是昆虫变形激素的前体.
- 这种相互作用凸显了昆虫-微生物共生在维持生态系统平衡方面的重要性.
研究的目的:
- 在其他无刺蜂物种中调查Zygosaccharomyces酵母共生菌的流行情况.
- 描述从蜜蜂繁殖细胞中分离出来的酵母菌株的ergosterol积累和形态.
- 为了确定Zygosaccharomyces属内的这些酵母分离物的遗传关系.
主要方法:
- 分析了来自巴西19种无刺蜂的繁殖细胞样本.
- 隔离和培养奥斯莫菲尔酵母,特别是Zygosaccharomyces spp. 的培养.
- 微镜检查酵母形态 (伪基形成) 和埃尔戈斯特醇积累.
- 用分子标记物对分离的酵母菌株进行了植物遗传学分析.
主要成果:
- 从八种不同的无刺蜂物种中分离出Zygosaccharomyces酵母.
- 隔离的酵母表现出伪菌的形成和埃尔戈斯特在脂质滴中的积累,反映了S. depilis的相互作用.
- 遗传学分析表明,这些与蜜蜂相关的酵母菌株代表了Zygosaccharomyces属内的新物种.
结论:
- 在Zygosaccharomyces酵母和多种无刺蜂物种之间存在着保存的共生相互作用.
- 鉴定出的酵母菌株很可能是新物种,这凸显了这种昆虫-微生物伙伴关系的特异性和进化意义.
- 这一发现扩大了我们对微生物共生体在社会昆虫生命周期和发展中的关键作用的理解.
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