干扰素刺激的基因15通过与NS1蛋白相互作用,促进BTV复制
Di Kang1,2, Guorui Zhang1, Zhonghui Zhang1
1State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Frontiers in microbiology
|August 28, 2023
概括
绵羊ISG15 (oISG15) 增强蓝舌病病毒 (BTV) 复制,独立于ISGylation. 它稳定病毒蛋白VP4和NS1,可能通过通过自途径干扰NS1降解.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 蓝舌病病毒 (BTV) 感染触发天生的免疫力,包括干扰素 (IFN) 和干扰素刺激基因 (ISG).
- ISG15是一种高度诱导的ISG,通常涉及抗病毒防御.
- 绵羊ISG15 (oISG15) 在BTV感染中的特定作用尚不清楚.
研究的目的:
- 研究oISG15在羊BTV感染期间的作用和分子机制.
- 为了确定oISG15是否促进或抑制BTV复制.
- 为了阐明oISG15和BTV病毒蛋白之间的相互作用.
主要方法:
- 定量实时PCR测量oISG15的转录.
- 过度表达和敲击实验,以评估oISG15对BTV复制的影响.
- 同免疫沉测定以确定相互作用的病毒蛋白和无处不在状态.
主要成果:
- oISG15的转录随时间和BTV剂量增加.
- 过度表达oISG15促进了BTV复制,而敲击抑制了它.
- oISG15促进了BTV复制,独立于其ISGylation功能.
- oISG15与BTV蛋白VP3,VP4,VP5和NS1.1发生相互作用.
- oISG15稳定了VP4和NS1,并通过自途径干扰了NS1降解.
结论:
- oISG15通过一种独立于ISGylation的机制促进BTV复制.
- oISG15与多个BTV蛋白相互作用,影响它们的稳定性.
- 这种相互作用,特别是与NS1,可能涉及调节自途径,为BTV-主机相互作用提供新的见解.
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