微生物从工程化伪马氏体中的芳香化合物中生产cis,cis-酸
Siyang He1, Weiwei Wang1, Weidong Wang2
1State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
Synthetic and systems biotechnology
|August 28, 2023
概括
这项研究利用Pseudomonas细菌将芳香污染物转化为cis,cis-muconate (ccMA). 这种生物道策略有效地将复杂的碳化合物转化为有价值的化合物.
科学领域:
- 环境微生物学环境微生物学
- 生物技术是生物技术.
- 生物修复是一种生物修复.
背景情况:
- 工业活动释放芳香碳化合物,造成环境污染.
- Pseudomonas 种类具有降解各种芳香化合物的代谢能力.
- 开发高效的生物催化剂对于生物修复和生物转化至关重要.
研究的目的:
- 为了设计Pseudomonas菌株的"生物道"战略.
- 为了将复杂的芳香碳化合物转化为cis,cis-muconate (ccMA).
- 使用工程化Pseudomonas putida B6-2和P. brassicacearum MPDS作为生物催化剂.
主要方法:
- 对 Pseudomonas 菌株进行基因改造,以增强芳香化合物的降解.
- 使用工程菌株B6-2 (B6-2ΔcatBΔsalC) 和MPDS (MPDSΔsalC ((pUCP18k-catA)) 的基因.
- 使用单一和混合的芳香基质,包括双,纳,酸,酸盐,和.
主要成果:
- 工程菌株实现了ccMA从双 (95.3%) 和纳 (100%) 的高摩尔转化.
- 再组合菌株通过插入外源基因集群成功将和转化为ccMA.
- 工程菌株的联盟证明了从混合芳香基质中高效的ccMA生产,达到85.2%的转化率.
结论:
- "生物道"战略有效地将芳香化合物转化为ccMA.
- 工程 Pseudomonas 菌株显示出生物修复和生产有价值化学品的巨大潜力.
- 这种方法提供了一种可持续的复杂污染物再利用方法.
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