使用受保护的支气管刷毛样本进行下一代元基因组测序的高特异性用于诊断儿童肺炎
Chunyan Zhang1,2, Zheng Li1,2, Mengyuan Wang1,2
1Department of Microbiology Laboratory, Children's Hospital Affiliated to Shandong University, Jinan, China.
Frontiers in cellular and infection microbiology
|August 28, 2023
概括
保护性支气管刷毛代遗传学下一代测序 (mNGS) 为诊断儿科肺炎提供了比支气管支气管洗液mNGS更高的特异性. 这种先进的病原体检测方法有望改善患有下呼吸道感染的儿童的临床结果.
科学领域:
- 儿科肺病学 儿科肺病学
- 传染性疾病 传染性疾病
- 分子诊断学 分子诊断学
背景情况:
- 下呼吸道感染是全球儿童疾病和死亡的主要原因之一.
- 准确和快速的病原体鉴定对于有效治疗儿科肺炎至关重要.
- 传统的诊断方法往往缺乏效率和特异性,阻碍了及时和精确的诊断.
研究的目的:
- 为了评估下一代元基因组测序 (mNGS) 的诊断性能,使用支气管洗液 (BALF) 和肺炎儿童的保护支气管刷 (BB) 样本.
- 为了将mNGS的疗效与检测病原体的常规测试 (CTs) 进行比较.
主要方法:
- 对1,032例儿科肺炎病例的回顾性分析.
- 在BALF和BB样本上对mNGS的性能评估.
- 与传统诊断试验结果进行比较.
主要成果:
- 与BALF mNGS (38.5%) 相比,受保护的支气管刷牙mNGS的特异性明显更高 (67.3%).
- BB mNGS (77.6%) 的整体巧合率最高,其次是BALF mNGS (76.5%) 和CTs (38.5%).
- 检测率因年龄组和病原体类型而异,与抗微生物治疗和症状发病相比,mNGS时间的具体建议.
结论:
- 这项研究是首次大规模评估保护支气管刷术mNGS用于诊断儿科肺部感染.
- 这些发现表明,BB mNGS是诊断儿童肺炎的高效方法,需要广泛的临床应用.
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