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从血中分离和隔离CD9阳性细胞外囊泡,使用流细胞计

Karan Khanna¹, Nikki Salmond¹, Sina Halvaei¹

¹Faculty of Pharmaceutical Sciences, The University of British Columbia Vancouver V6T 1Z3 Canada karla.williams@ubc.ca.

Nanoscale advances

|August 28, 2023

概括

流细胞计有效地从血等复杂样本中分离纳米级的细胞外囊泡 (EV). 这种方法可以直接隔离特定的EV亚群,有助于发现用于疾病检测的生物标志物.

科学领域:

生物技术是生物技术.
纳米技术纳米技术
分析化学分析化学

背景情况:

细胞外囊泡 (EVs) 是疾病检测的关键生物标志物,但由于它们的小尺寸和复杂的生物基质,难以分离.
目前对电动汽车的隔离方法通常是繁的,可能无法有效地去除污染颗粒和蛋白质.

研究的目的:

评估流式细胞计对隔离纳米级粒子,特别是细胞外囊泡 (EVs) 的实用性.
通过流细胞测量来评估CD9阳性EVs从血中直接分离的情况.

主要方法:

使用100nm纳米粒子和~124nm病毒校准流细胞计,以建立纳米级分类能力.
该方法被应用于直接从人体血中分类CD9阳性EV,而无需进行预富步骤.

主要成果:

流细胞测量证明了纳米尺寸尺寸范围内的粒子的成功分类.
通过流式细胞计量,有效地从血中分离出CD9阳性EV,无论是从预先丰富的分数,还是直接从样本中分离出来.

结论:

流细胞计是一种可行的技术,用于隔离纳米级的细胞外囊泡 (EVs).
这种方法简化了从等离子体等复杂的生物流体中隔离EV,促进了基于EV的生物标志物研究和诊断.

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