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在伊朗使用下一代测序对与Helicoverpa armigera相关的内共生细菌进行基因组鉴定

Parinaz Sheibani¹, Manizheh Jamshidi¹, Reza Khakvar²

¹Department of Plant Protection, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

Bioinformatics and biology insights

|August 28, 2023

概括

这项研究确定了两个内共生细菌,Buchnera aphidicola和Serratia symbiotica,与破坏性农业害虫Helicoverpa armigera有关. 这种基因组表征提供了对这种国际化昆虫的潜在生物控制策略的见解.

科学领域:

农业昆虫学农业昆虫学
微生物基因组学微生物基因组学
昆虫与微生物的相互作用

背景情况:

螺旋 (Helicoverpa armigera) 是一个主要的世界性农业害虫,损害了120多种植物.
了解害虫的内部微生物群对于识别弱点和潜在的生物控制剂至关重要.

研究的目的:

为了进行与Helicoverpa armigera相关的微生物社区的基因组表征.
为了识别H. armigera幼虫中的内共生细菌.

主要方法:

使用Illumina平台对H. armigera幼虫的全基因组测序.
使用MetaPhlAn2管道进行生物信息分析.
细菌基因组的对齐和组装.

主要成果:

测序产生了大约2GB的原始数据.
确定了两个内共生细菌物种,即Buchnera aphidicola和Serratia symbiotica.
组装了完整的基因组:B. aphidicola (657,623 bp, 27.4% GC) 和S. symbiotica (1,595,135 bp, 42.90% GC).它们的基因组分别是:

结论:

这项研究提供了首份关于Buchnera aphidicola和Serratia symbiotica作为Helicoverpa armigera中的内共生体的全球报告.
这些发现有助于了解H. armigera的生物学,并为害虫管理提供了途径.

关键词:

布奇纳虫虫 (Buchnera aphidicola) 是一种虫.这是一架直升飞机的直升机pa armigera.伊朗伊朗伊朗伊朗没有NGS,没有NGS.这种植物是Serratia symbiotica.

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