Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

804
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
804

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

IL-17A deficiency in HLA-DR3 transgenic mice enriches beneficial Prevotella species in gut to promote Tregs and reduce CNS autoimmunity.

Microbiome·2026
Same author

Tumor-draining lymph nodes in ovarian cancer lack germinal centers but harbor tumor-reactive memory B cells clonally linked to intra-tumoral B cells.

Immunity·2026
Same author

Highly focused human CD8+ T-cell response in the lower airways during acute influenza infection.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Pre-transplant TCR Network Topology Predicts Kidney Allograft Rejection Independent of HLA Mismatch.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: Biallelic variants in the noncoding RNA gene RNU4-2 cause a recessive neurodevelopmental syndrome with distinct white matter changes.

Nature genetics·2026
Same author

Saturation editing of RNU4-2 reveals distinct dominant and recessive disorders.

Nature·2026
Same journal

Editorial: Technologies for RNA Detection.

Bio-protocol·2026
Same journal

One-Step Affinity Purification of MarathonRT Reverse Transcriptase for RNA Sequencing Applications.

Bio-protocol·2026
Same journal

Enhanced RNA-Seq Expression Profiling and Functional Enrichment in Non-model Organisms Using Custom Annotations.

Bio-protocol·2026
Same journal

Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell Types.

Bio-protocol·2026
Same journal

Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data.

Bio-protocol·2026
Same journal

Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture.

Bio-protocol·2026
查看所有相关文章

相关实验视频

Updated: Jul 17, 2025

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
05:17

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Published on: May 14, 2019

8.6K

使用单细胞RNA测序和参考地图进行T细胞克隆分析.

Massimo Andreatta1,2,3, Paul Gueguen1,2,3, Nicholas Borcherding4

  • 1Ludwig Institute for Cancer Research, Lausanne Branch, and Department of Oncology, CHUV and University of Lausanne, Epalinges, Switzerland.

Bio-protocol
|August 28, 2023
PubMed
概括
此摘要是机器生成的。

本研究介绍了一个计算工作流程,用于分析T细胞状态和克隆类型,使用单细胞RNA测序 (scRNA-seq) 和T细胞受体 (TCR) 数据. 该方法将T细胞克隆性与它们的转录态联系起来,揭示了克隆扩张和功能表型之间的关系.

关键词:
参考投影的参考投影是一个指标.一个单细胞分析.一个T细胞克隆.这种T细胞受体是T细胞受体.在TCR中,可以使用TCR.文字转录学 (Transcriptomics) 是一个学科.这就是 scRNA-seqq.在 scTCR-seqq 中使用.

更多相关视频

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.3K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.6K

相关实验视频

Last Updated: Jul 17, 2025

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
05:17

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Published on: May 14, 2019

8.6K
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.3K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.6K

科学领域:

  • 免疫学 免疫学 免疫学
  • 计算生物学 计算生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • T细胞利用T细胞受体 (TCR) 进行抗原识别,从而实现适应性免疫反应.
  • 独特的TCR序列作为跟踪T细胞克隆类型的分子条形码.
  • 单细胞RNA测序 (scRNA-seq) 提供结合的TCR和转录数据,用于详细的T细胞分析.

研究的目的:

  • 从scRNA-seq数据中呈现综合T细胞状态和克隆分析的计算工作流.
  • 为了使T细胞功能状态和克隆结构的特征化.
  • 将T细胞克隆性与转录基因特征联系起来,以获得更深入的生物学见解.

主要方法:

  • 使用R包Seurat,ProjecTILs和scRepertoire进行数据分析.
  • 使用ProjecTILs的参考投影用于自动化的T细胞状态注释.
  • 集成scRNA-seq和TCR测序数据,用于全面的克隆型追踪.

主要成果:

  • 工作流允许对T细胞功能状态进行自动注释.
  • 能够详细跟踪单个T细胞克隆类型,包括扩张和增殖.
  • 有助于评估不同T细胞亚型之间的克隆重叠.
  • 将克隆扩张和分化状态与功能表型联系起来.

结论:

  • 提出的计算工作流提供了一种可复制的方法来分析T细胞状态和克隆性.
  • 这种方法通过整合转录和TCR目录数据来增强对T细胞反应的理解.
  • 这些发现支持使用scRNA-seq和计算工具来剖析适应性免疫.