带有双层对齐的微通道的脱细胞化支架诱导膀光滑肌细胞的定向生长:向尿道和尿道重建
Quhan Cheng1, Linli Zhang1, Jingai Zhang1
1Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China.
Advanced healthcare materials
|August 28, 2023
概括
工程尿道组织支架模仿本地双向光滑肌肉细胞 (SMC) 的对齐. 具有对齐的微通道的来自老鼠的dECM支架促进SMC生长和功能,用于潜在的尿道和尿管再生.
科学领域:
- 生物材料工程 生物材料工程
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 尿道组织,如尿道和尿管,由于临床需要需要再生.
- 原生尿道的特点是双向对齐的光滑肌肉细胞 (SMCs),对于功能至关重要.
- 目前的尿路支架缺乏诱导本地模仿SMC对齐的能力.
研究的目的:
- 设计模管脱细胞化外细胞矩阵 (dECM) 支架,模仿本地尿道组织结构.
- 用双向对齐的微通道和完整的内部层创建支架,以支持SMC再生.
- 评估这些dECM支架在尿道和尿道重建方面的潜力.
主要方法:
- 使用模板方法制造管状dECM支架,采用皮下植入的模板方法.
- 通过调整模板纤维直径来设计具有双向对齐微通道的dECM支架.
- 在实验室中评估脚手架的性能,包括泄漏压力,机械强度和SMC对齐.
主要成果:
- 设计的dECM管状支架具有完整的内部层,增强了泄漏压力.
- 具有大约65微米微通道的来自老鼠的dECM支架显示出合适的机械性能.
- 这些支架有效地诱导了人类膀SMC的双向对齐和生长,具有高的功能蛋白表达.
结论:
- 来自老鼠的管状dECM支架与双层对齐的微通道显示了组织工程的希望.
- 这些支架可能有助于在尿路重建中模仿平滑肌肉细胞的本地再生.
- 工程支架代表了在开发功能性尿道组织替代品方面取得的重大进展.
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